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Sample GSM254429 Query DataSets for GSM254429
Status Public on May 08, 2008
Title GM9017, unaffectd brother
Sample type RNA
 
Source name GM9017
Organism Homo sapiens
Characteristics unaffectd brother
Biomaterial provider Corielle
Growth protocol B-Cells from Corielle were incubated at 37oC and 5% CO2 overnight, the day after cells are counted. Cells were expanded in RPMI 1640 with 2mM L-glutamine and 15% FBS following Corielle's lymphoblast line maintenance protocols. 1x10^7 cells were spun down and resuspended in 2 ml Tri-Reagent and frozen overnight at -80oC prior to RNA extraction
Extracted molecule total RNA
Extraction protocol Std Tri-Reagent protocol followed by cleanup on RNEasy columns using an on-column DNase step. RNA quality is checked using a Nanodrop and an Agilent Bioanalyzer
Label biotin-11-UTP
Label protocol Standard Codelink protocol
 
Hybridization protocol Standard Codelink protocol
Scan protocol Standard Codelink protocol on Axon genePix 4000B
Description GM9017, unaffected brother, 45yrs, family 2112
Data processing Codelink Expression Analysis Softwarev4.2
 
Submission date Jan 03, 2008
Last update date May 08, 2008
Contact name Sridar V Chittur
E-mail(s) schittur@albany.edu
Phone 518-591-7215
Organization name SUNY-University at Albany
Department Center for Functional Genomics
Lab Microarray Core
Street address One Discovery Drive, CRC 342G
City Rensselaer
State/province NY
ZIP/Postal code 12144
Country USA
 
Platform ID GPL2895
Series (1)
GSE10064 Gene expression in immortalized B-lymphocytes derived from patients with multiple sclerosis

Data table header descriptions
ID_REF
VALUE NORMALIZED EXPRESSION VALUE
EXPRESSIONVALUE RAW EXPRESSION VALUE
GENESPRINGFLAG P=Present, A=Absent
CODELINKFLAG L=Low signal, G=Good signal
SPOT_COL Spot column
SPOT_ROW Spot row
SPOTMEAN
SPOTMEDIAN
BKGMEAN
BKGMEDIAN

Data table
ID_REF VALUE EXPRESSIONVALUE GENESPRINGFLAG CODELINKFLAG SPOT_COL SPOT_ROW SPOTMEAN SPOTMEDIAN BKGMEAN BKGMEDIAN
1002 10.39841056 643.916687 P G 2 1 703.916687 717 63.46703339 60
1003 0.059385539 3.67742157 A L 3 1 64.67742157 67 62.22527313 61
1004 0.010335151 0.63999939 A L 4 1 57.63999939 53 61.79069901 57
1005 0.12181294 7.543209076 A CL 5 1 61.54320908 56 72.97080231 54
1006 0.254293192 15.74698639 A L 6 1 71.74698639 64 61.70984268 56
1007 0.288071738 17.83870697 A L 7 1 82.83870697 66 67.06111145 65
1009 1.464056501 90.66101074 P G 9 1 147.6610107 143 59.43125153 57
1010 10.06232089 623.1044922 P G 10 1 678.1044922 698 57.69403076 55
1011 0.116726988 7.228263855 A L 11 1 68.22826385 57 62.05789566 61
1012 1.424544993 88.21427917 P G 12 1 146.2142792 135 63.08024597 58
1013 0.128420471 7.952377319 A L 13 1 67.95237732 65 67.38710022 60
1014 0.121022397 7.494255066 A L 14 1 64.49425507 62 62.60326004 57
1016 1.043875666 64.64151001 P G 16 1 123.64151 114 62.21977997 59
1017 0.100790087 6.241378784 A L 17 1 66.24137878 66 62.15753555 60
1018 0.235105904 14.55882263 A L 18 1 80.55882263 77 67.46575165 66
1019 0.309429769 19.16129303 A L 19 1 75.16129303 68 62.10344696 56
1020 0.182480239 11.30000305 A L 20 1 67.30000305 58 60.34513092 56
1021 7.190203943 445.25 P G 21 1 506.25 552 62.29670334 61
1023 3.053377214 189.078949 P G 23 1 248.078949 250 61.04026794 59
1024 0.17541913 10.86274719 A L 24 1 68.86274719 68 62.11320877 58

Total number of rows: 54167

Table truncated, full table size 3751 Kbytes.




Supplementary file Size Download File type/resource
GSM254429.tif.gz 44.6 Mb (ftp)(http) TIFF
Processed data included within Sample table

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