|
| Status |
Public on Jan 09, 2008 |
| Title |
S. cerevisiae SMP_Treated_C |
| Sample type |
RNA |
| |
|
| Source name |
S. cerevisiae Strain S288C cells treated with sampangine (SMP)
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
S. cerevisiae S288C cells at OD 0.2 were treated with sampangine (SMP) at IC50 concentration (1.17 ug/ml), allowed to grow to OD 0.5, then harvested and frozen. Three biological replicate samples were generated.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy Midi-kit
|
| Label |
Biotin
|
| Label protocol |
Affymetrix One Cycle Target Labeling kit
|
| |
|
| Hybridization protocol |
Affymetrix GenChip Hybridization protocol, and GeneChip Fluidics Station 450 default protocol
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 default parameters
|
| Description |
S. cerevisiae SMP_Treated_C
|
| Data processing |
Image analysis, scaling (TGT = 500), and probeset-level data analysis using default parameters in GCOS v1.1
|
| |
|
| Submission date |
Jan 04, 2008 |
| Last update date |
Jan 08, 2008 |
| Contact name |
Ameeta Agarwal |
| E-mail(s) |
aagarwal@olemiss.edu
|
| Phone |
662-915-1218
|
| Organization name |
University of Mississippi
|
| Department |
National Center for Natural Products Research
|
| Street address |
NCNPR, Room 2049
|
| City |
University |
| State/province |
MS |
| ZIP/Postal code |
38677 |
| Country |
USA |
| |
|
| Platform ID |
GPL90 |
| Series (2) |
| GSE10073 |
Gene expression response to the antifungal compound sampangine in S. cerevisiae |
| GSE10104 |
Gene expression response to the antifungal compound sampangine |
|
