|
| Status |
Public on Jan 09, 2008 |
| Title |
C. albicans SMP_Tr_Vs_Un_B |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
C.albicans SC5314 cells treated with solvent (0.25% DMSO)
|
| Organism |
Candida albicans |
| Characteristics |
C. albicans SC5314 cells at OD 0.2 were treated with solvent (0.25% DMSO), allowed to grow to OD 0.5, then harvested and frozen. Three biological replicate samples were generated.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy Midi-kit
|
| Label |
Cy3
|
| Label protocol |
Aminoallyl labeling protocol from The Institute for Genomic Research (www.tigr.com)
|
| |
|
| Channel 2 |
| Source name |
C.albicans SC5314 cells treated with sampangine (SMP)
|
| Organism |
Candida albicans |
| Characteristics |
C. albicans SC5314 cells at OD 0.2 were treated with sampangine (SMP) at IC50 concentration (6.13 ug/ml), allowed to grow to OD 0.5, then harvested and frozen. Three biological replicate samples were generated.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy Midi-kit
|
| Label |
Cy5
|
| Label protocol |
Aminoallyl labeling protocol from The Institute for Genomic Research (www.tigr.com)
|
| |
|
| |
| Hybridization protocol |
Hybridization and washing protocols from the Corning UltraGAPS Coated Slides Instruction Manual (www.corning.com/lifesciences)
|
| Scan protocol |
Default parameters on ScanArray 5000 scanner (Perkin Elmer)
|
| Description |
C. albicans SMP Treated vs. Untreated B
|
| Data processing |
Image analysis was performed using QuantArray v3.0 software (Perkin Elmer). Differentially expressed genes were identified using default parameters of BRB Array Tools v3.2.3 software developed by Dr. Richard Simon and Amy Peng Lam (http://linus.nci.nih.gov/BRB-ArrayTools.html). Data normalization was done using Lowess algorithm available in BRB ArrayTools.
|
| |
|
| Submission date |
Jan 06, 2008 |
| Last update date |
Jan 08, 2008 |
| Contact name |
Ameeta Agarwal |
| E-mail(s) |
aagarwal@olemiss.edu
|
| Phone |
662-915-1218
|
| Organization name |
University of Mississippi
|
| Department |
National Center for Natural Products Research
|
| Street address |
NCNPR, Room 2049
|
| City |
University |
| State/province |
MS |
| ZIP/Postal code |
38677 |
| Country |
USA |
| |
|
| Platform ID |
GPL6346 |
| Series (2) |
| GSE10075 |
Gene expression response to the antifungal compound sampangine in C. albicans |
| GSE10104 |
Gene expression response to the antifungal compound sampangine |
|
