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Sample GSM257431 Query DataSets for GSM257431
Status Public on Dec 22, 2008
Title REF colon repl 4
Sample type RNA
 
Source name human colon
Organism Homo sapiens
Characteristics healthy control reference sample
batch 5
Extracted molecule total RNA
Extraction protocol RNeasy Plus Mini kit (QIAGEN, Hilden, Germany)
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA amplified with TargetAmp 1-round Aminoallyl-aRNA Amplication Kit 101 (Epicentre, WI) (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on the human HG-U133 Plus 2.0 GeneChip array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix Genechip Scanner 3000.
Description gene expression data from human colon
Data processing The data were analyzed with GeneSpring version 7.3 using RMA normalization with a custom CDF file(Hs133P_Hs_REFSEQ_8) , then normalized to the internal control for each batch and the mean of the healthy control reference samples.
 
Submission date Jan 16, 2008
Last update date Dec 22, 2008
Contact name Lee Denson
E-mail(s) lee.denson@cchmc.org
Organization name CCHMC
Street address 3333 Burnet Avenue
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platform ID GPL5760
Series (1)
GSE10191 Human colon expression in healthy controls and UC

Data table header descriptions
ID_REF
VALUE data was RMA normalized, then normalized to the internal control sample for each batch and the mean of the healthy control reference samples

Data table
ID_REF VALUE
NM_000014_at 1.4667689
NM_000015_at 1.3195559
NM_000016_at 1.0457288
NM_000017_at 0.58077323
NM_000018_at 0.5954321
NM_000019_at 1.1432116
NM_000020_at 0.83568496
NM_000021_at 0.93856376
NM_000022_at 0.701198
NM_000023_at 1.2704948
NM_000024_at 1.1869129
NM_000025_at 0.8880815
NM_000026_at 1.1793308
NM_000027_at 1.6598889
NM_000028_at 0.9683969
NM_000029_at 1.7058936
NM_000030_at 0.97274524
NM_000031_at 1.0242602
NM_000032_at 0.90518796
NM_000033_at 0.8821144

Total number of rows: 26121

Table truncated, full table size 596 Kbytes.




Supplementary file Size Download File type/resource
GSM257431.CEL.gz 5.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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