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Sample GSM257437 Query DataSets for GSM257437
Status Public on Dec 22, 2008
Title REF colon repl 10
Sample type RNA
 
Source name human colon
Organism Homo sapiens
Characteristics healthy control reference sample
batch 1
Extracted molecule total RNA
Extraction protocol RNeasy Plus Mini kit (QIAGEN, Hilden, Germany)
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA amplified with TargetAmp 1-round Aminoallyl-aRNA Amplication Kit 101 (Epicentre, WI) (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on the human HG-U133 Plus 2.0 GeneChip array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix Genechip Scanner 3000.
Description gene expression data from human colon
Data processing The data were analyzed with GeneSpring version 7.3 using RMA normalization with a custom CDF file(Hs133P_Hs_REFSEQ_8) , then normalized to the internal control for each batch and the mean of the healthy control reference samples.
 
Submission date Jan 16, 2008
Last update date Dec 22, 2008
Contact name Lee Denson
E-mail(s) lee.denson@cchmc.org
Organization name CCHMC
Street address 3333 Burnet Avenue
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platform ID GPL5760
Series (1)
GSE10191 Human colon expression in healthy controls and UC

Data table header descriptions
ID_REF
VALUE data was RMA normalized, then normalized to the internal control sample for each batch and the mean of the healthy control reference samples

Data table
ID_REF VALUE
NM_000014_at 0.54763585
NM_000015_at 0.5128656
NM_000016_at 0.75615823
NM_000017_at 0.8961007
NM_000018_at 1.2252196
NM_000019_at 0.8759476
NM_000020_at 0.71099436
NM_000021_at 0.7860632
NM_000022_at 1.6985781
NM_000023_at 0.75317085
NM_000024_at 0.69764274
NM_000025_at 0.9380162
NM_000026_at 1.1978222
NM_000027_at 0.77724487
NM_000028_at 0.789962
NM_000029_at 0.42733654
NM_000030_at 0.8615978
NM_000031_at 0.9820474
NM_000032_at 0.9697711
NM_000033_at 0.7545412

Total number of rows: 26121

Table truncated, full table size 598 Kbytes.




Supplementary file Size Download File type/resource
GSM257437.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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