EGF treatment was performed on cultures by supplementing EGF at 10ng/ml for 72 hours prior to harvesting
Growth protocol
Both PMC42-ET and the PMC42-LA sublines were grown in RPMI, supplemented with 10% FCS and penicillin/streptomycin, at 370C/10% CO2.
Extracted molecule
genomic DNA
Extraction protocol
cells were washed with 1 x PBS, dispersed by trypsin digestion, and the cell pellets were lysed in RIPA buffer. Lysates where then digested with proteinase K, followed by phenol-chloroform extraction and isopropanol precipitation
Label
Cy5 and Cy3
Label protocol
As per Illumina protocol
Hybridization protocol
As per Illumina protocol
Scan protocol
As per Illumina protocol
Description
LAS1
Data processing
Data analysed using R and Bioconductor with the ChAMP Library v2.6.0
