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Sample GSM2592455 Query DataSets for GSM2592455
Status Public on Jul 31, 2018
Title MTF2 shRNA clone 7 replicate 1
Sample type SRA
 
Source name cord blood
Organism Homo sapiens
Characteristics cell type: Lin(-)CD34+ sorted
Treatment protocol HSPCs (Lin - CD34 + ) were transduced either with the GFP-tagged MTF2 shRNA clone or a scrambled shRNA control. The transduced GFP + cells were sorted 72 hours post transduction using a MoFlo sorter. RNA was extracted from 150,000 cells per condition for RNA-seq analysis.
Extracted molecule polyA RNA
Extraction protocol RNA was isolated (Arcturus PicoPure Kit, LifeTech) and Dnase treated (Qiagen).
Iillumina TruSeq Library Prep Kit
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Data processing Replicate data was analyzed using TopHat v1.4.1 and Cuffdiff 1.3.0
DAVID analysis of signficantly
Cytoscape Enrichment Map visualization
Genome_build: hg19, Ensembl 67
Supplementary_files_format_and_content: gene_exp.diff
 
Submission date Apr 28, 2017
Last update date May 15, 2019
Contact name William Stanford
E-mail(s) wstanford@ohri.ca
Phone 613-737-8899
Organization name Ottawa Hospital Research Institute
Lab Stanford
Street address 501 Smyth Road
City Ottawa
State/province Ontario
ZIP/Postal code K1H 8L6
Country Canada
 
Platform ID GPL11154
Series (2)
GSE98343 Targeting the MTF2-MDM2 Axis Sensitizes Refractory Acute Myeloid Leukemia to Chemotherapy [RNA-Seq]
GSE98380 Targeting the MTF2-MDM2 Axis Sensitizes Refractory Acute Myeloid Leukemia to Chemotherapy
Relations
BioSample SAMN06854467
SRA SRX2771322

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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