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Sample GSM2611564 Query DataSets for GSM2611564
Status Public on Jun 30, 2017
Title ChIP-seq_BRD4_Mutu_100E6_vehicle_Replicate2
Sample type SRA
Source name MutuI (EBV+ Burkitt lymphoma cell line)
Organisms Homo sapiens; human gammaherpesvirus 4
Characteristics cell line: MutuI
pathogen: Epstein-Barr virus
chip antibody: BRD4
Treatment protocol Unless noted, cells were not treated. For Mutu ChIPs with 100E6 cells, cells were treated with either 1 uM JQ1 or vehicle for 24 hours, and then cells were fixed for ChIP.
Extracted molecule genomic DNA
Extraction protocol Unless noted, 18E6 cells were collected for ChIP. For the Mutu cells treated with JQ1 or vehicle, 100E6 cells were collected for ChIP.
DNA was prepared and ChIP performed as described in the paper
ChIP-seq libraries were prepared as described in the paper
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 4000
Data processing 50 bp reads were mapped using Bowtie to an index containing both the human hg19 and EBV reference [GenBank ID: NC_007605.1] genomes.
Parameters allowed for up to 2 mismatches and 2 alignments
Peaks were visualized after normalization to the background baseline
Formatted for uploading to Integrative Genomics Viewer (
Genome_build: index contains both the human hg19 and EBV reference [GenBank ID: NC_007605.1] genomes
Supplementary_files_format_and_content: Wigs are formatted for IGV. Genome file chrEBV(B95_8_Raji).fa is available from Wig columns are chromosome, start, stop, counts per million mapped reads.
Submission date May 10, 2017
Last update date May 15, 2019
Contact name JJ Miranda
Organization name Barnard College, Columbia University
Street address 3009 Broadway
City New York
ZIP/Postal code 10027
Country USA
Platform ID GPL23362
Series (2)
GSE84213 BET proteins bind the lytic origins of replication [MutuI ChIP-seq]
GSE84214 BET inhibitors block the Epstein-Barr virus lytic cycle at two distinct steps
BioSample SAMN06927282
SRA SRX2795364

Supplementary file Size Download File type/resource
GSM2611564_Mutu_100_veh_Brd4_ChIPseq_rep2.igv.wig.gz 1.0 Mb (ftp)(http) WIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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