|
| Status |
Public on Jul 15, 2017 |
| Title |
12hr ZIKV-infected human brain microvascular endothelial cells |
| Sample type |
RNA |
| |
|
| Source name |
ZIKV-infected_hBMECs_12hr-post infection
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: human brain microvascular endothelial cells (hBMECs)
passage: passage 5
infected with: Zka virus (ZIKV) at an MOI 20
time point: 12hr-post infection
|
| Treatment protocol |
mock-infected or ZIKV infected at an MOI 20
|
| Growth protocol |
human brain microvascular endothelial cells passage 5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cell were lysed in Qiagen RLT Buffer and total RNA was purified using Qiagen Rneasy kit.
|
| Label |
biotin
|
| Label protocol |
Fragmented and labeled single-stranded cDNA was generated from total RNA using the GeneChip WT PLUS Reagent Kit ( Affymetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized to GeneChip Human Clariom S Arrays (Affymetrix) using the Hydridization, Wash and Stain kit (Affymetrix). .
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix). Arrays were scanned at the Stony Brook Genomics Core Facility
|
| Description |
12hr PRV
|
| Data processing |
Raw data was processed with the Expression Console software (Affymetrix) for background correction and normalization. Data analysis and statistical evaluations were performed with Transcriptome Analysis Console (TAC) Software (Affymetrix)
affymetrix-algorithm-name = sst-rma-gene-full
affymetrix-algorithm-version = 1.0
program-name = Expression Console
program-version = 1.4.1.46
|
| |
|
| Submission date |
May 12, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Erich R Mackow |
| E-mail(s) |
erich.mackow@stonybrook.edu
|
| Phone |
6316327014
|
| Organization name |
Stony Brook University
|
| Department |
Molecular Genetics & Microbiology
|
| Lab |
Mackow Laboratory
|
| Street address |
100 Nicholls Road
|
| City |
Stony Brook |
| State/province |
NY |
| ZIP/Postal code |
11794 |
| Country |
USA |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE98889 |
Zka virus (ZIKV) persistently infects primary human brain microvascular endothelial cells |
|
