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Sample GSM2643475 Query DataSets for GSM2643475
Status Public on Feb 23, 2018
Title PbIP5d1(WT_5d_1)
Sample type SRA
Source name C2C12
Organism Mus musculus
Characteristics cell line: C2C12
time point: 5 day
genotype: wild type
Extracted molecule total RNA
Extraction protocol RNA was harvested using Trizol reagent.TruSeq® RNA LT Sample Prep Kit v2 was used with total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
Description PbIP5d1
Data processing Illumina Hiseq3000 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm9 whole genome using STAR software
Fragments Per Kilobase of exon per Megabase of library size (FPKM) were calculated. In short, exons from all isoforms of a gene were merged to create one meta-transcript. The number of reads falling in the exons of this meta-transcript were counted and normalized by the size of the meta-transcript and by the size of the library.
Genome_build: mm9
Supplementary_files_format_and_content: tab-delimited text files include the expression values of this transcript for each Sample
Submission date May 30, 2017
Last update date May 15, 2019
Contact name Kan He
Organization name Anhui University
Street address 111 Jiulong Road
City Hefei
State/province Anhui
ZIP/Postal code 230601
Country China
Platform ID GPL21493
Series (1)
GSE99399 A transcriptomic study of myogenic differentiation under the overexpression of PPARγ by RNA-Seq
BioSample SAMN07176076
SRA SRX2867576

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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