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Sample GSM2645627 Query DataSets for GSM2645627
Status Public on Oct 06, 2017
Title Malignant lymphoma 6
Sample type genomic
 
Channel 1
Source name C57BL/6 mouse normal kidney
Organism Mus musculus
Characteristics strain: C57BL/6
cell type: normal kidney
Treatment protocol The treated groups initially consisted of 20 male mice for each. Ferric nitrilotriacetate was injected intraperitoneally 5 times a week for 12 weeks. The administration was started at a dose of 3 mg iron/mouse kg weight for the first week. And the dose was increased to 5 mg iron/mouse kg weight for the next 11 weeks. The mice were euthanized at ~120 weeks.
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using DNeasy Blood & Tissue Kit (QIAGEN).
Label Cy3
Label protocol The extracted genomic DNA was labeled using Aagilent Sure tag DNA labeling kit according to the manufacturer's protocol (Version 7.1).
 
Channel 2
Source name ML_WT1121
Organism Mus musculus
Characteristics strain: C57BL/6
cell type: Malignant lymphoma
genotype: Mutyh wild type
treatment: Fe-NTA 3 mo
Treatment protocol The treated groups initially consisted of 20 male mice for each. Ferric nitrilotriacetate was injected intraperitoneally 5 times a week for 12 weeks. The administration was started at a dose of 3 mg iron/mouse kg weight for the first week. And the dose was increased to 5 mg iron/mouse kg weight for the next 11 weeks. The mice were euthanized at ~120 weeks.
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using DNeasy Blood & Tissue Kit (QIAGEN).
Label Cy5
Label protocol The extracted genomic DNA was labeled using Aagilent Sure tag DNA labeling kit according to the manufacturer's protocol (Version 7.1).
 
 
Hybridization protocol The labeled DNA was hybridized with Agilent SurePrint G3 Mouse CGH 4x180k microarray at 65°C for 24 hours according to the manufacturer's protocol (Version 7.1).
Scan protocol The slides were scanned in an Agilent DNA microarray scanner with SureScan High-Resolution Technology (G2565CA).
Description ML_WT1121
Data processing The scanned images were analyzed with Agilent Feature Extraction Software 10.7 using default parameters (CGH_107_Sep09 protocol).
 
Submission date Jun 01, 2017
Last update date Jan 23, 2018
Contact name Shinya Toyokuni
E-mail(s) akatsuka@med.nagoya-u.ac.jp
Organization name Nagoya University
Department Pathology
Street address 65 Tsuruma-Cho, Showa-Ku
City Nagoya
State/province Aichi
ZIP/Postal code 466-8550
Country Japan
 
Platform ID GPL10449
Series (1)
GSE99535 Genomic profiles of oxidative stress-induced renal cell carcinomas developed in Mutyh deficient mice

Data table header descriptions
ID_REF
VALUE Normalized signal log2 ratio [Cy5/Cy3]

Data table
ID_REF VALUE
23 0.16160963
24 0.036020853
25 0.009525251
26 0.08753529
27 0.23017775
28 0.07275304
29 0.29453906
30 0.030969834
31 0.101978414
32 -0.053199958
33 0.29238525
34 0.08014345
35 0.3108232
36 0.2525593
37 0.1484063
38 0.20219506
39 0.21691602
40 0.41774088
41 0.06343209
42 0.113918915

Total number of rows: 174303

Table truncated, full table size 3083 Kbytes.




Supplementary file Size Download File type/resource
GSM2645627_ML_WT1121.txt.gz 50.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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