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Sample GSM2645629 Query DataSets for GSM2645629
Status Public on Oct 06, 2017
Title Malignant lymphoma 8
Sample type genomic
 
Channel 1
Source name C57BL/6 mouse normal kidney
Organism Mus musculus
Characteristics strain: C57BL/6
cell type: normal kidney
Treatment protocol The treated groups initially consisted of 20 male mice for each. Ferric nitrilotriacetate was injected intraperitoneally 5 times a week for 12 weeks. The administration was started at a dose of 3 mg iron/mouse kg weight for the first week. And the dose was increased to 5 mg iron/mouse kg weight for the next 11 weeks. The mice were euthanized at ~120 weeks.
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using DNeasy Blood & Tissue Kit (QIAGEN).
Label Cy3
Label protocol The extracted genomic DNA was labeled using Aagilent Sure tag DNA labeling kit according to the manufacturer's protocol (Version 7.1).
 
Channel 2
Source name ML_WT1420
Organism Mus musculus
Characteristics strain: C57BL/6
cell type: Malignant lymphoma
genotype: Mutyh wild type
treatment: untreated
Treatment protocol The treated groups initially consisted of 20 male mice for each. Ferric nitrilotriacetate was injected intraperitoneally 5 times a week for 12 weeks. The administration was started at a dose of 3 mg iron/mouse kg weight for the first week. And the dose was increased to 5 mg iron/mouse kg weight for the next 11 weeks. The mice were euthanized at ~120 weeks.
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using DNeasy Blood & Tissue Kit (QIAGEN).
Label Cy5
Label protocol The extracted genomic DNA was labeled using Aagilent Sure tag DNA labeling kit according to the manufacturer's protocol (Version 7.1).
 
 
Hybridization protocol The labeled DNA was hybridized with Agilent SurePrint G3 Mouse CGH 4x180k microarray at 65°C for 24 hours according to the manufacturer's protocol (Version 7.1).
Scan protocol The slides were scanned in an Agilent DNA microarray scanner with SureScan High-Resolution Technology (G2565CA).
Description ML_WT1420
Data processing The scanned images were analyzed with Agilent Feature Extraction Software 10.7 using default parameters (CGH_107_Sep09 protocol).
 
Submission date Jun 01, 2017
Last update date Jan 23, 2018
Contact name Shinya Toyokuni
E-mail(s) akatsuka@med.nagoya-u.ac.jp
Organization name Nagoya University
Department Pathology
Street address 65 Tsuruma-Cho, Showa-Ku
City Nagoya
State/province Aichi
ZIP/Postal code 466-8550
Country Japan
 
Platform ID GPL10449
Series (1)
GSE99535 Genomic profiles of oxidative stress-induced renal cell carcinomas developed in Mutyh deficient mice

Data table header descriptions
ID_REF
VALUE Normalized signal log2 ratio [Cy5/Cy3]

Data table
ID_REF VALUE
23 0.007114866
24 0.18940486
25 0.047589537
26 0.057132933
27 -0.013525147
28 0.20223339
29 0.25286153
30 0.002930656
31 0.1077888
32 0.20682275
33 0.10015213
34 -0.09914033
35 0.35411745
36 -0.014200015
37 0.18828262
38 -0.08352526
39 0.039315045
40 0.12960926
41 -0.10999853
42 0.07487013

Total number of rows: 174303

Table truncated, full table size 3079 Kbytes.




Supplementary file Size Download File type/resource
GSM2645629_ML_WT1420.txt.gz 50.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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