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Sample GSM264982 Query DataSets for GSM264982
Status Public on Mar 31, 2008
Title Primary lung fibroblasts, RNA expression, technical replicate A
Sample type RNA
 
Source name Primary lung fibroblasts
Organism Homo sapiens
Characteristics Total RNA
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from 107-2x107 cells using
RNAeasy Kit (QIAGEN) following the manufacturer’s instructions. RNA quality was ensured sing the Agilent Systems Bioanalyzer.
Label Biotin
Label protocol Biotinylated RNA was prepared with the Illumina TotalPrep RNA Amplification kit (Ambion)
 
Hybridization protocol 34ul of the Hyb mix with labeled cRNA sample was dispensed onto the center of each array and allowed to hyb with rotations in a pre-heated oven for 16-20h at 55*C. Washes were done with Wash E1BC solution, Block E1 solution was used for blocking and detection performed by Block E1 solution+Streptavidin-Cy3/5.
Scan protocol Standard Illumina procedure
Description no additional description
Data processing Rank invariant normalization method implemented in Bead Studio software
 
Submission date Feb 11, 2008
Last update date Mar 12, 2008
Contact name Vitalina Komashko
E-mail(s) vitalina.komashko@sagebase.org
Organization name Sage Bionetworks
Street address 1100 Fairview Ave N
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL6255
Series (1)
GSE10504 Using ChIP-chip technology to reveal common principles of transcriptional repression in normal and cancer cells

Data table header descriptions
ID_REF
VALUE rank invariant normalized expression value
DET_VALUE Illumina detection Pvalue

Data table
ID_REF VALUE DET_VALUE
ILMN_10000 202.002 0.001412429
ILMN_10001 4516.067 0
ILMN_10002 -37.31121 0.9576271
ILMN_10004 950.3939 0
ILMN_10005 66.15401 0.01553672
ILMN_10006 26.24402 0.1327684
ILMN_10009 41.32875 0.04378531
ILMN_1001 -21.32556 0.8121469
ILMN_10010 -17.8298 0.7711864
ILMN_10011 557.7018 0
ILMN_10012 -13.13459 0.700565
ILMN_10013 -0.5092412 0.4661017
ILMN_10014 28.09424 0.31842
ILMN_10016 38.8626 0.05649718
ILMN_1002 -16.341 0.740113
ILMN_10020 398.579 0
ILMN_10021 166.4736 0.001412429
ILMN_10022 26.30968 0.1327684
ILMN_10023 174.9466 0.001412429
ILMN_10024 8.776388 0.3079096

Total number of rows: 20589

Table truncated, full table size 557 Kbytes.




Supplementary file Size Download File type/resource
GSM264982.csv.gz 154.2 Kb (ftp)(http) CSV
GSM264982.idat.gz 1.1 Mb (ftp)(http) IDAT
GSM264982.jpg.gz 959.8 Kb (ftp)(http) JPG
GSM264982.txt.gz 11.2 Mb (ftp)(http) TXT
GSM264982.xml.gz 660 b (ftp)(http) XML
Processed data included within Sample table

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