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Sample GSM264984 Query DataSets for GSM264984
Status Public on Mar 31, 2008
Title Primary lung fibroblasts, RNA expression, technical replicate F
Sample type RNA
 
Source name Primary lung fibroblasts
Organism Homo sapiens
Characteristics Total RNA
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from 107-2x107 cells using
RNAeasy Kit (QIAGEN) following the manufacturer’s instructions. RNA quality was ensured sing the Agilent Systems Bioanalyzer.
Label Biotin
Label protocol Biotinylated RNA was prepared with the Illumina TotalPrep RNA Amplification kit (Ambion)
 
Hybridization protocol 34ul of the Hyb mix with labeled cRNA sample was dispensed onto the center of each array and allowed to hyb with rotations in a pre-heated oven for 16-20h at 55*C. Washes were done with Wash E1BC solution, Block E1 solution was used for blocking and detection performed by Block E1 solution+Streptavidin-Cy3/5.
Scan protocol Standard Illumina procedure
Description no additional description
Data processing Rank invariant normalization method implemented in Bead Studio software
 
Submission date Feb 11, 2008
Last update date Mar 12, 2008
Contact name Vitalina Komashko
E-mail(s) vitalina.komashko@sagebase.org
Organization name Sage Bionetworks
Street address 1100 Fairview Ave N
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL6255
Series (1)
GSE10504 Using ChIP-chip technology to reveal common principles of transcriptional repression in normal and cancer cells

Data table header descriptions
ID_REF
VALUE rank invariant normalized expression value
DET_VALUE Illumina detection Pvalue

Data table
ID_REF VALUE DET_VALUE
ILMN_10000 204.9786 0.001412429
ILMN_10001 4349.237 0
ILMN_10002 -10.58604 0.6793785
ILMN_10004 961.2207 0
ILMN_10005 113.9752 0.005649718
ILMN_10006 21.81048 0.1836158
ILMN_10009 69.64633 0.01412429
ILMN_1001 3.221188 0.4237288
ILMN_10010 -12.38635 0.7033898
ILMN_10011 530.5306 0
ILMN_10012 -9.585609 0.6652542
ILMN_10013 20.3366 0.200565
ILMN_10014 14.34434 0.3614902
ILMN_10016 21.86698 0.1836158
ILMN_1002 -12.22099 0.7033898
ILMN_10020 365.3539 0
ILMN_10021 198.8079 0.001412429
ILMN_10022 -18.47303 0.8064972
ILMN_10023 204.3201 0.001412429
ILMN_10024 6.29404 0.3841808

Total number of rows: 20589

Table truncated, full table size 555 Kbytes.




Supplementary file Size Download File type/resource
GSM264984.csv.gz 153.9 Kb (ftp)(http) CSV
GSM264984.idat.gz 1.1 Mb (ftp)(http) IDAT
GSM264984.jpg.gz 955.3 Kb (ftp)(http) JPG
GSM264984.txt.gz 11.2 Mb (ftp)(http) TXT
GSM264984.xml.gz 668 b (ftp)(http) XML
Processed data included within Sample table

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