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Sample GSM266908 Query DataSets for GSM266908
Status Public on Nov 28, 2008
Title U937 cells transfected with empty pSGMtNeo vector - Replica 1
Sample type RNA
 
Source name U937 myelomoncytic cell line, transfected with pSGMtNeo vector
Organism Homo sapiens
Characteristics U937 cell line (human cell line established from a diffuse histiocytic lymphoma and displaying monocytic characteristics) transfected with pSGMtNeo vector treated for 8h with 100µM ZnSO4.
Treatment protocol Cell lines were treated for 8h with 100uM ZnSO4 prior to RNA extraction.
Growth protocol U937 cell lines were maintained in RPMI-1640 supplemented with 10% FCS and 2mM glutamine at 37°C in a humidified atmosphere containing 5% CO2.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TRIzol Reagent (Gibco), followed by clean up on RNeasy mini/midi columns (RNeasy Mini/Midi Kit, Qiagen). For each cell line, an RNA pool was obtained by mixing equal quantities of total RNA from each of the three independent RNA extractions. First strand cDNA synthesis was performed on 15ugr of total RNA with Superscript III (Invitrogen, USA) using Oligo(dT)12–18 Primer according to manufacturer’s protocol. Complementary second strand was then synthesized and 4ug of doublestranded cDNAs were shipped to NimbleGen Services for labeling, hybridization, and scanning.
Label Cy3
Label protocol Doublestranded cDNA was random-prime labeled with Cy3-nonamers.
 
Hybridization protocol Hybridization is performed for 16 - 20 hours at 42°C.
Scan protocol NimbleGen arrays are scanned with a GenePix 4000B Scanner.
Description U937 cells transfected with empty pSGMtNeo vector
Data processing None
 
Submission date Feb 20, 2008
Last update date Nov 05, 2008
Contact name Myriam Alcalay
E-mail(s) myriam.alcalay@ieo.eu
Organization name European Institute of Oncology
Department Experimental Oncology
Lab Functional Genomics
Street address Via Adamello 16
City Milan
ZIP/Postal code 20139
Country Italy
 
Platform ID GPL6510
Series (2)
GSE10537 Gene expression profile and DNA binding pattern of AML1/ETO in U937 cells
GSE10579 Analysis of expression levels of genes on chromosome 19 in U937 cells expressing AML1/ETO

Data table header descriptions
ID_REF
VALUE Signal intensity (raw)

Data table
ID_REF VALUE
CHR1900P000011001 5914.89
CHR1900P000011050 982.22
CHR1900P000011099 3468.00
CHR1900P000011148 4942.78
CHR1900P000011197 5795.33
CHR1900P000011246 7528.33
CHR1900P000011295 26601.22
CHR1900P000011344 8222.56
CHR1900P000011393 30737.55
CHR1900P000011442 19809.22
CHR1900P000011658 14150.78
CHR1900P000011707 8287.00
CHR1900P000011756 5109.22
CHR1900P000011805 8343.56
CHR1900P000011854 5665.11
CHR1900P000011903 50038.45
CHR1900P000011952 52682.22
CHR1900P000012001 19086.55
CHR1900P000012050 47961.22
CHR1900P000012099 56720.45

Total number of rows: 385796

Table truncated, full table size 9503 Kbytes.




Supplementary data files not provided
Processed data not provided for this record

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