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Status |
Public on Nov 28, 2008 |
Title |
U937 cells transfected with empty pSGMtNeo vector - Replica 1 |
Sample type |
RNA |
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|
Source name |
U937 myelomoncytic cell line, transfected with pSGMtNeo vector
|
Organism |
Homo sapiens |
Characteristics |
U937 cell line (human cell line established from a diffuse histiocytic lymphoma and displaying monocytic characteristics) transfected with pSGMtNeo vector treated for 8h with 100µM ZnSO4.
|
Treatment protocol |
Cell lines were treated for 8h with 100uM ZnSO4 prior to RNA extraction.
|
Growth protocol |
U937 cell lines were maintained in RPMI-1640 supplemented with 10% FCS and 2mM glutamine at 37°C in a humidified atmosphere containing 5% CO2.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using TRIzol Reagent (Gibco), followed by clean up on RNeasy mini/midi columns (RNeasy Mini/Midi Kit, Qiagen). For each cell line, an RNA pool was obtained by mixing equal quantities of total RNA from each of the three independent RNA extractions. First strand cDNA synthesis was performed on 15ugr of total RNA with Superscript III (Invitrogen, USA) using Oligo(dT)12–18 Primer according to manufacturer’s protocol. Complementary second strand was then synthesized and 4ug of doublestranded cDNAs were shipped to NimbleGen Services for labeling, hybridization, and scanning.
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Label |
Cy3
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Label protocol |
Doublestranded cDNA was random-prime labeled with Cy3-nonamers.
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|
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Hybridization protocol |
Hybridization is performed for 16 - 20 hours at 42°C.
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Scan protocol |
NimbleGen arrays are scanned with a GenePix 4000B Scanner.
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Description |
U937 cells transfected with empty pSGMtNeo vector
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Data processing |
None
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Submission date |
Feb 20, 2008 |
Last update date |
Nov 05, 2008 |
Contact name |
Myriam Alcalay |
E-mail(s) |
myriam.alcalay@ieo.eu
|
Organization name |
European Institute of Oncology
|
Department |
Experimental Oncology
|
Lab |
Functional Genomics
|
Street address |
Via Adamello 16
|
City |
Milan |
ZIP/Postal code |
20139 |
Country |
Italy |
|
|
Platform ID |
GPL6510 |
Series (2) |
GSE10537 |
Gene expression profile and DNA binding pattern of AML1/ETO in U937 cells |
GSE10579 |
Analysis of expression levels of genes on chromosome 19 in U937 cells expressing AML1/ETO |
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