|
| Status |
Public on Dec 31, 2017 |
| Title |
Recurrence_Endom Cancer_2 |
| Sample type |
RNA |
| |
|
| Source name |
Endometrium cancer
|
| Organism |
Homo sapiens |
| Characteristics |
recurrence: Recurrence +
sample origin: FPPE section
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the miRNeasy FFPE Kit (Qiagen, Courtaboeuf, France), according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
After validation of the RNA quality with Bioanalyzer 2100, 1 µg of total RNA was biotin labelled following the FlashTag Biotin HSR RNA labelling kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, mirRNA targets were hybridized for 20 hr at 48C on mirRNA-40 Affymetrix. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GCS 3000 scanner (Affymetrix)
|
| Data processing |
The data were preprocessed and analyzing using Partek Genomic Suite and the customed cdf brain Array V21 (http://brainarray.mbni.med.umich.edu/Brainarray/Database/CustomCDF/21.0.0/mirbasef.asp) with mirID as identifiers.
|
| |
|
| Submission date |
Jun 15, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Céline Méhats |
| E-mail(s) |
celine.mehats@inserm.fr
|
| Organization name |
Inserm
|
| Department |
U1016
|
| Street address |
24 rue du Faubourg St jacques
|
| City |
Paris |
| ZIP/Postal code |
75014 |
| Country |
France |
| |
|
| Platform ID |
GPL23587 |
| Series (1) |
| GSE100078 |
Identification of micro-RNA expression profile related to recurrence in women with ESMO low-risk endometrial cancer |
|
