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Sample GSM2670897 Query DataSets for GSM2670897
Status Public on Dec 31, 2017
Title Recurrence_Endom Cancer_3
Sample type RNA
 
Source name Endometrium cancer
Organism Homo sapiens
Characteristics recurrence: Recurrence +
sample origin: FPPE section
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the miRNeasy FFPE Kit (Qiagen, Courtaboeuf, France), according to the manufacturer's instructions.
Label biotin
Label protocol After validation of the RNA quality with Bioanalyzer 2100, 1 µg of total RNA was biotin labelled following the FlashTag Biotin HSR RNA labelling kit (Affymetrix).
 
Hybridization protocol Following fragmentation, mirRNA targets were hybridized for 20 hr at 48C on mirRNA-40 Affymetrix. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GCS 3000 scanner (Affymetrix)
Data processing The data were preprocessed and analyzing using Partek Genomic Suite and the customed cdf brain Array V21 (http://brainarray.mbni.med.umich.edu/Brainarray/Database/CustomCDF/21.0.0/mirbasef.asp) with mirID as identifiers.
 
Submission date Jun 15, 2017
Last update date Jan 23, 2018
Contact name Céline Méhats
E-mail(s) celine.mehats@inserm.fr
Organization name Inserm
Department U1016
Street address 24 rue du Faubourg St jacques
City Paris
ZIP/Postal code 75014
Country France
 
Platform ID GPL23587
Series (1)
GSE100078 Identification of micro-RNA expression profile related to recurrence in women with ESMO low-risk endometrial cancer

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
HAIRPIN_hsa-let-7a-1_at 1.8055
HAIRPIN_hsa-let-7a-2_at 2.2037
HAIRPIN_hsa-let-7a-3_at 1.59446
HAIRPIN_hsa-let-7b_at 2.01346
HAIRPIN_hsa-let-7c_at 1.77822
HAIRPIN_hsa-let-7d_at 3.13753
HAIRPIN_hsa-let-7e_at 1.79404
HAIRPIN_hsa-let-7f-1_at 1.58784
HAIRPIN_hsa-let-7f-2_at 1.19892
HAIRPIN_hsa-let-7g_at 1.74684
HAIRPIN_hsa-let-7i_at 1.52431
HAIRPIN_hsa-mir-100_at 2.76632
HAIRPIN_hsa-mir-101-1_at 1.42368
HAIRPIN_hsa-mir-101-2_at 1.4905
HAIRPIN_hsa-mir-103a-1_at 1.1677
HAIRPIN_hsa-mir-103a-2_at 1.23294
HAIRPIN_hsa-mir-103b-1_at 1.15386
HAIRPIN_hsa-mir-103b-2_at 1.22238
HAIRPIN_hsa-mir-105-1_at 1.29477
HAIRPIN_hsa-mir-105-2_at 1.27707

Total number of rows: 4458

Table truncated, full table size 141 Kbytes.




Supplementary file Size Download File type/resource
GSM2670897_R+3_LREC-84D.CEL.gz 768.5 Kb (ftp)(http) CEL
Processed data included within Sample table

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