|
| Status |
Public on Aug 20, 2017 |
| Title |
RNA-seq_Col_rep1 |
| Sample type |
SRA |
| |
|
| Source name |
Seedlings, WT
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col
genotype/variation: wildtype
tissue: seedlings
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using an RNeasy plus mini kit (Qiagen).
RNA-seq libraries were generated from total RNA with a TruSeq RNA library prep kit (Illumina) following the manufacturer’s instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Reads were aligned to the Arabidopsis genome (TAIR10) using TopHat.
Reads per gene were counted by HTSeq.
Reads per gene were normalized and compared with DESeq2.
Genome_build: TAIR10
Supplementary_files_format_and_content: *.txt: Tab-delimited text files include read counts.
|
| |
|
| Submission date |
Jul 11, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Danhua Jiang |
| E-mail(s) |
dhjiang@genetics.ac.cn
|
| Organization name |
Institute of Genetics and Developmental Biology
|
| Street address |
No.1 West Beichen Road
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
|
| Platform ID |
GPL17639 |
| Series (2) |
| GSE101219 |
DNA replication-coupled histone modification maintains Polycomb gene silencing in plants [RNA-seq] |
| GSE101221 |
DNA replication-coupled histone modification maintains Polycomb gene silencing in plants |
|
| Relations |
| BioSample |
SAMN07343956 |
| SRA |
SRX2996419 |
