|
| Status |
Public on Mar 07, 2008 |
| Title |
MCFDCIS+RASF T2 Exp 2 35 days |
| Sample type |
genomic |
| |
|
| Source name |
MCFDCIS+RASF T2 Exp1 xenograft
|
| Organism |
Homo sapiens |
| Characteristics |
none
|
| Growth protocol |
Cells were grown in the media recommended by the providers. MCFDCIS cells were injected subcutaneously into 6-9 week old female nude mice alone or together with 2-3 fold excess of HME, RASF, PBS, or PBTS cells in 50% Matrigel. Tumors were allowed to grow for 3-8 weeks.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
We use the guanidinium thiocyanate/cesium chloride centrifugation method to isolate RNA/DNA from large amounts of tissue/cells and the mMACS mRNA isolation kit for small amounts (less than 1 million cells). The DNA fraction was extracted with PC8, treated with proteinase K and isopropanol precipitated.
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
SNP array hybridizations were performed by the Dana-Farber Microarray Core using Affymetrix 11K XbaI or 250K StyI SNP arrays and protocols recommended by Affymetrix
|
| Scan protocol |
Arrays were scanned using a confocal laser scanner, and Affymetrix genotyping software (Affymetrix GeneChip 5.0) was used to make allele calls for all loci.
|
| Description |
MCFDCIS+RASF T2 Exp 2 35 days
|
| Data processing |
Data were then analyzed using dChip or genepattern software.
|
| |
|
| Submission date |
Mar 06, 2008 |
| Last update date |
Mar 06, 2008 |
| Contact name |
Kornelia Polyak |
| E-mail(s) |
kornelia_polyak@dfci.harvard.edu
|
| Phone |
617-632-2106
|
| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Medical Oncology
|
| Lab |
Polyak
|
| Street address |
450 Brookline Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL1266 |
| Series (1) |
| GSE10747 |
Analysis of genetic changes in MCF10A series cells and MCFDCIS derived xenografts |
|
