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Status |
Public on Sep 21, 2010 |
Title |
Affymetrix expression in hrp1 1 |
Sample type |
RNA |
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Source name |
delta-hrp1 strain
|
Organism |
Schizosaccharomyces pombe |
Characteristics |
Strain: delta-hrp1. Cells were grown to mid-log phase in rich medium.
|
Biomaterial provider |
Paulina Wanrooij
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated from mid-log phase cultures according to Xue et al. 2004 (Yeast 21(1):25-39).
|
Label |
biotin
|
Label protocol |
Labeling was performed according to the GeneChip expression analysis technical manual that is available for download at www.affymetrix.com.
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|
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Hybridization protocol |
According to the GeneChip expression analysis technical manual that is available for download at www.affymetrix.com.
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Scan protocol |
According to the GeneChip expression analysis technical manual that is available for download at www.affymetrix.com.
|
Description |
Gene expression in the delta-hrp1 S. pombe strain was analysed using the Affymetrix GeneChip Yeast Genome 2.0 array.
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Data processing |
After the ExpressionStat algorithm, data was imported into Genespring GX (Agilent technologies), per-gene normalized to median and filtered on flags.
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Submission date |
Mar 10, 2008 |
Last update date |
Sep 21, 2010 |
Contact name |
Claes M Gustafsson |
E-mail(s) |
claes.gustafsson@medkem.gu.se
|
Organization name |
Gothenburg University
|
Department |
Department of Medical biochemistry and cell biology
|
Street address |
Medcinaregatan 9A
|
City |
Gothenburg |
ZIP/Postal code |
405 30 |
Country |
Sweden |
|
|
Platform ID |
GPL2529 |
Series (1) |
GSE10079 |
A Med15 - Hrp1 complex associates with fission yeast Mediator |
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