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Sample GSM27230 Query DataSets for GSM27230
Status Public on Nov 01, 2005
Title ceg1.1
Sample type RNA
 
Channel 1
Source name OY94 (wt) @ 39 deg
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name OY163 (ceg1-250) @ 39 deg
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description Reference and mutant strains grown at 30oC to 1-2 x 10exp7 cells/ml. shifted to 39 deg for 45 minutes. RNA extracted.
gpr file # 53
 
Submission date Jul 22, 2004
Last update date May 28, 2005
Contact name Todd A Burckin
E-mail(s) burckin@biology.ucsc.edu
Phone 831 459-5245
Fax 831 459-3139
Organization name University of California Santa Cruz
Department Molecular, Cell, & Developmental Biology
Lab Hartzog Lab
Street address 1156 High Street
City Santa Cruz
State/province CA
ZIP/Postal code 95062
Country USA
 
Platform ID GPL1370
Series (1)
GSE1610 Analyzing the role of transcription elongation factors Spt4-5 in splicing

Data table header descriptions
ID_REF
X X-coordinate of spot
Y Y-coordinate of spot
Dia. spot diameter in microns
F635 Median Channel 2 (Cy5/635) median pixel intensity - feature
F635 Mean Channel 2 (Cy5/635) mean pixel intensity - feature
F635 SD Channel 2 (Cy5/635) standard deviation of pixel intensities - feature
B635 Median Channel 2 (Cy5/635) median background intensity
B635 Mean Channel 2 (Cy5/635) mean background intensity
B635 SD Channel 2 (Cy5/635) standard deviation of background
% > B635+1SD Channel 2 (Cy5/635) percent of pixels 1 StDev above background
% > B635+2SD Channel 2 (Cy5/635) percent of pixels 2 StDevs above background
F635 % Sat. Channel 2 (Cy5/635) percent of pixels saturated
F532 Median Channel 1 (Cy3/532) median pixel intensity - feature
F532 Mean Channel 1 (Cy3/532) mean pixel intensity - feature
F532 SD Channel 1 (Cy3/532) standard deviation of pixel intensities - feature
B532 Median Channel 1 (Cy3/532) median background intensity
B532 Mean Channel 1 (Cy3/532) mean background intensity
B532 SD Channel 1 (Cy3/532) standard deviation of background
% > B532+1SD Channel 1 (Cy3/532) percent of pixels 1 StDev above background
% > B532+2SD Channel 1 (Cy3/532) percent of pixels 2 StDevs above background
F532 % Sat. Channel 1 (Cy3/532) percent of pixels saturated
Ratio of Medians (635/532)
Ratio of Means (635/532)
Median of Ratios (635/532)
Mean of Ratios (635/532)
Ratios SD (635/532)
Rgn Ratio (635/532)
Rgn R? (635/532)
F Pixels Number of feature pixels
B Pixels Number of background pixels
Sum of Medians Sum of medians
Sum of Means Sum of means
VALUE Log ratio (base 2)
F635 Median - B635 Channel 2 (Cy5/635) background subtracted median feature intensity
F532 Median - B532 Channel 1 (Cy3/532) background subtracted median feature intensity
F635 Mean - B635 Channel 2 (Cy5/635) background subtracted mean feature intensity
F532 Mean - B532 Channel 1 (Cy3/532) background subtracted mean feature intensity
F635 Total Intensity F635 Total Intensity
F532 Total Intensity F532 Total Intensity
SNR 635 n/a
SNR 532 n/a
Flags User defined flag (default=0)
Normalize n/a

Data table
ID_REF X Y Dia. F635 Median F635 Mean F635 SD B635 Median B635 Mean B635 SD % > B635+1SD % > B635+2SD F635 % Sat. F532 Median F532 Mean F532 SD B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians (635/532) Ratio of Means (635/532) Median of Ratios (635/532) Mean of Ratios (635/532) Ratios SD (635/532) Rgn Ratio (635/532) Rgn R? (635/532) F Pixels B Pixels Sum of Medians Sum of Means VALUE F635 Median - B635 F532 Median - B532 F635 Mean - B635 F532 Mean - B532 F635 Total Intensity F532 Total Intensity SNR 635 SNR 532 Flags Normalize
1 2550 25870 90 50 107 169 34 34 5 84 69 0 2147 2889 1693 30 31 6 100 100 0 0.008 0.026 0.05 0.052 5.247 0.01 0.029 52 369 2133 2932 -7.048 16 2117 73 2859 5569 150246 14.6 476.333 0 0
2 2730 25880 70 69 90 49 34 34 5 96 87 0 154 174 94 30 30 3 100 100 0 0.282 0.389 0.284 0.317 2.853 0.297 0.332 32 230 159 200 -1.825 35 124 56 144 2887 5568 11.2 48 0 0
3 2900 25870 80 66 84 58 34 34 5 100 94 0 89 97 42 30 31 3 100 100 0 0.542 0.746 0.528 0.571 1.573 1.014 0.816 52 321 91 117 -0.883 32 59 50 67 4373 5069 10 22 0 0
4 3080 25880 110 218 240 89 34 34 5 100 100 0 146 154 45 30 30 3 100 100 0 1.586 1.661 1.626 1.645 1.184 1.731 0.949 80 472 300 330 0.666 184 116 206 124 19216 12331 41.2 41.333 0 0
5 3260 25880 100 108 136 98 35 36 6 100 100 0 174 177 42 31 31 4 100 100 0 0.51 0.692 0.527 0.569 1.528 0.979 0.689 80 426 216 247 -0.97 73 143 101 146 10865 14140 16.667 36.5 0 0
6 3440 25890 110 23119 23416 5560 36 36 7 100 100 0 18159 17573 3488 31 31 4 100 100 0 1.273 1.333 1.293 1.326 1.134 1.34 0.978 80 433 41211 40922 0.349 23083 18128 23380 17542 1873315 1405831 3340 4385.5 0 0
7 3600 25880 100 4677 4318 992 35 36 7 100 100 0 6232 5685 1348 31 31 3 100 100 0 0.749 0.758 0.764 0.77 1.217 0.754 0.982 80 415 10843 9937 -0.418 4642 6201 4283 5654 345474 454836 611.714 1884.667 0 0
8 3790 25890 110 1052 1042 84 35 35 5 100 100 0 1316 1313 147 31 31 3 100 100 0 0.791 0.785 0.784 0.788 1.099 0.779 0.982 80 444 2302 2289 -0.337 1017 1285 1007 1282 83336 105027 201.4 427.333 0 0
9 3950 25890 110 1585 1461 422 35 35 6 100 100 0 11059 10202 3487 31 31 4 100 100 0 0.141 0.14 0.14 0.143 1.188 0.136 0.978 80 443 12578 11597 -2.831 1550 11028 1426 10171 116917 816176 237.667 2542.75 0 0
10 4130 25900 110 962 964 191 34 34 5 100 100 0 651 630 140 30 31 4 100 100 0 1.494 1.55 1.553 1.571 1.186 1.569 0.961 80 482 1549 1530 0.58 928 621 930 600 77105 50375 186 149.75 0 0
11 4310 25910 120 695 672 297 33 33 4 100 100 0 687 701 370 31 31 3 99 99 0 1.009 0.954 0.98 1.104 1.673 0.908 0.965 120 478 1318 1309 0.013 662 656 639 670 80610 84099 159.75 223.333 0 0
12 4480 25910 110 435 444 101 33 33 4 100 100 0 1185 1256 382 31 32 7 100 100 0 0.348 0.336 0.344 0.341 1.215 0.321 0.951 80 427 1556 1636 -1.521 402 1154 411 1225 35529 100516 102.75 174.857 0 0
13 4650 25910 110 489 499 128 32 33 7 100 100 0 316 324 85 30 31 7 100 100 0 1.598 1.588 1.617 1.591 1.116 1.616 0.949 80 460 743 761 0.676 457 286 467 294 39940 25957 66.571 41.857 0 0
14 4830 25920 110 102 107 36 33 34 23 96 82 0 95 99 26 30 33 29 96 68 0 1.062 1.072 1.056 1.024 1.388 1.135 0.891 80 488 134 143 0.086 69 65 74 69 8526 7940 3.174 2.276 0 0
15 5010 25920 100 183 183 53 32 33 5 100 100 0 1060 985 242 30 30 3 100 100 0 0.147 0.158 0.148 0.155 1.35 0.152 0.869 80 413 1181 1106 -2.77 151 1030 151 955 14622 78817 30 318.333 0 0
16 5170 25910 90 93 98 33 32 33 5 100 100 0 200 203 33 30 30 3 100 100 0 0.359 0.382 0.353 0.35 1.526 0.389 0.786 52 350 231 239 -1.479 61 170 66 173 5117 10541 13 57.667 0 0
17 5350 25920 90 58 83 56 32 32 4 98 92 0 87 96 39 29 30 3 100 100 0 0.448 0.761 0.543 0.547 1.866 1.017 0.818 52 376 84 118 -1.158 26 58 51 67 4319 4983 12.75 22 0 0
18 5530 25920 90 50 74 54 32 33 4 84 73 0 58 72 34 30 30 3 100 100 0 0.643 1 0.683 0.609 2.422 1.332 0.799 52 376 46 84 -0.637 18 28 42 42 3831 3731 10.25 14 0 0
19 5710 25930 90 91 113 54 32 33 4 100 100 0 334 339 33 30 30 3 100 100 0 0.194 0.262 0.19 0.224 1.642 0.248 0.611 52 351 363 390 -2.365 59 304 81 309 5867 17623 20 103 0 0
20 5870 25910 90 67 88 56 33 34 5 100 100 0 102 108 32 30 30 3 100 100 0 0.472 0.705 0.522 0.547 1.615 0.942 0.703 52 364 106 133 -1.082 34 72 55 78 4571 5622 10.8 26 0 0

Total number of rows: 5760

Table truncated, full table size 1068 Kbytes.




Supplementary data files not provided

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