|
| Status |
Public on Apr 25, 2008 |
| Title |
sample 16a _ SNP _ CALL-preB without t(9;22) |
| Sample type |
genomic |
| |
|
| Source name |
White blood human cells
|
| Organism |
Homo sapiens |
| Characteristics |
Leukemia samples
Bone Marrow sample
|
| Treatment protocol |
White blood cells were isolated through Ficoll-Hypaque density gradient centrifugation
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Lysis of the mononuclear cells followed by lysate homonogenization using a biopolymer shredding system in a microcentrifuge spin-column format followed by total RNA purification using selective binding columns
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 11 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450DX.
|
| Scan protocol |
GeneChips were scanned using the Scan GCS3000Dx.
|
| Description |
NK_ALL_1782_Hind
SNP arrays data from pediatric acute leukemia samples at diagnosis
|
| Data processing |
The data were analyzed using R software [www.r-project.org] with BioConductor package [www.bioconductor.org]
|
| |
|
| Submission date |
Mar 11, 2008 |
| Last update date |
Mar 11, 2008 |
| Contact name |
Andrea Zangrando |
| E-mail(s) |
andrea.zangrando@unipd.it
|
| Organization name |
University of Padova
|
| Department |
Woman and Child's Health
|
| Lab |
Hemato-Oncology
|
| Street address |
Via Giustiniani, 3
|
| City |
Padova |
| ZIP/Postal code |
35138 |
| Country |
Italy |
| |
|
| Platform ID |
GPL2004 |
| Series (1) |
| GSE10792 |
Genome wide genotyping and gene expression data of childhood B-cell precursor ALL without known genetic aberrations |
|
