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Sample GSM2732499 Query DataSets for GSM2732499
Status Public on Aug 05, 2017
Title Liver_Scap_WT_chipC_rep2
Sample type RNA
 
Source name Liver, wild type mouse
Organism Mus musculus
Characteristics genotype: wild type
age: 10-12 weeks
Sex: Male
diet: Teklad chow
tissue: Liver
exposure time: 14 days
Treatment protocol Livers were excised for RNA extraction
Growth protocol Mice were housed in colony cages in rooms maintained on a 12-h light/12-h dark cycle.
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from each liver by using RNA STAT-60 (Tel-Test, Friendswood, TX)
Label biotin
Label protocol Equal aliquots of total RNA from each of five mouse livers in each group were pooled (total, 20 μg) and used for biotin labeling as described in the Affymetrix technical bulletin
 
Hybridization protocol Hybridization and washing of the Affymetrix GeneChip Murine Genome MU74A, -B, and -C version 2 arrays (Affymetrix, Santa Clara, CA) were carried out as described in: Mahadevappa, M. & Warrington, J. A. (1999) Nat. Biotechnol. 17, 1134–1136. Duplicate hybridizations were performed with each RNA sample.
Scan protocol Scanning, and analysis of the Affymetrix GeneChip Murine Genome MU74A, -B, and -C version 2 arrays (Affymetrix, Santa Clara, CA) were carried out as described in: Mahadevappa, M. & Warrington, J. A. (1999) Nat. Biotechnol. 17, 1134–1136.
Description Mice received four i.p. injections of polyinosinic-polycytidylic acid to induce the Cre recombinase and euthanized 14 days after last injection
Data processing Gene expression results were analyzed by standard methods using Robust Multichip Average (RMA) normalization.
 
Submission date Aug 04, 2017
Last update date Jan 23, 2018
Contact name Christopher Corton
E-mail(s) corton.chris@epa.gov
Organization name US-EPA
Lab MD B105-01
Street address 109 TW Alexander Dr.
City Research Triangle Park
State/province NC
ZIP/Postal code 27711
Country USA
 
Platform ID GPL83
Series (2)
GSE102258 Combined analysis of oligonucleotide microarray data from transgenic and knockout mice identifies direct SREBP target genes [MG_U74Cv2]
GSE102259 Combined analysis of oligonucleotide microarray data from transgenic and knockout mice identifies direct SREBP target genes

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
128520_at 9.16174105825978
128523_at 2.80125863402897
128529_at 4.36010133644065
128550_at 5.45523538992913
128554_at 5.05419780985485
128577_s_at 4.45839420245887
128578_r_at 3.49445968235555
128579_r_at 4.5115373759028
128580_at 5.8937739540673
128582_at 4.75717066741254
128583_at 4.07321987278798
128590_at 5.03688099860153
128624_at 3.69922806888979
128643_r_at 7.92801441452175
128644_at 6.87999681747953
128645_at 5.5758812445099
128646_at 4.62543552305417
128647_at 6.78973253462007
128651_at 6.81940294961892
128652_at 4.27421271294608

Total number of rows: 11934

Table truncated, full table size 322 Kbytes.




Supplementary file Size Download File type/resource
GSM2732499_Norma_WT_C.CEL.gz 2.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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