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Sample GSM2732503 Query DataSets for GSM2732503
Status Public on Aug 05, 2017
Title Liver_Human_Transgenic_SREBP1a_chipC_rep2
Sample type RNA
 
Source name Liver, transgenic SREBP-1a mouse
Organism Mus musculus
Characteristics genotype: TgSREBP-1a
age: 12-16 weeks
Sex: Male
diet: high protein/low carbohydrate
tissue: Liver
exposure time: 2 weeks
Treatment protocol Livers were excised for RNA extraction
Growth protocol Mice were housed in colony cages in rooms maintained on a 12-h light/12-h dark cycle.
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from each liver by using RNA STAT-60 (Tel-Test, Friendswood, TX)
Label biotin
Label protocol Equal aliquots of total RNA from each of five mouse livers in each group were pooled (total, 20 μg) and used for biotin labeling as described in the Affymetrix technical bulletin
 
Hybridization protocol Hybridization and washing of the Affymetrix GeneChip Murine Genome MU74A, -B, and -C version 2 arrays (Affymetrix, Santa Clara, CA) were carried out as described in: Mahadevappa, M. & Warrington, J. A. (1999) Nat. Biotechnol. 17, 1134–1136. Duplicate hybridizations were performed with each RNA sample.
Scan protocol Scanning, and analysis of the Affymetrix GeneChip Murine Genome MU74A, -B, and -C version 2 arrays (Affymetrix, Santa Clara, CA) were carried out as described in: Mahadevappa, M. & Warrington, J. A. (1999) Nat. Biotechnol. 17, 1134–1136.
Description Mice were fed a high protein/low carbohydrate diet for 2 weeks. This diet was used to elicit high-level expression of the nSREBPs
Data processing Gene expression results were analyzed by standard methods using Robust Multichip Average (RMA) normalization.
 
Submission date Aug 04, 2017
Last update date Jan 23, 2018
Contact name Christopher Corton
E-mail(s) corton.chris@epa.gov
Organization name US-EPA
Lab MD B105-01
Street address 109 TW Alexander Dr.
City Research Triangle Park
State/province NC
ZIP/Postal code 27711
Country USA
 
Platform ID GPL83
Series (2)
GSE102258 Combined analysis of oligonucleotide microarray data from transgenic and knockout mice identifies direct SREBP target genes [MG_U74Cv2]
GSE102259 Combined analysis of oligonucleotide microarray data from transgenic and knockout mice identifies direct SREBP target genes

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
128520_at 9.18709973331461
128523_at 2.94741403988162
128529_at 4.29722295972358
128550_at 5.59512482414702
128554_at 5.21684466687968
128577_s_at 4.30507694220693
128578_r_at 3.49196971950542
128579_r_at 4.72522816412769
128580_at 5.91148968592509
128582_at 4.97805350643423
128583_at 4.24753436609434
128590_at 5.03982783261907
128624_at 3.82560639746366
128643_r_at 8.02835584757177
128644_at 7.03823682224708
128645_at 5.65954248921133
128646_at 4.62435799150247
128647_at 6.34891705705397
128651_at 7.03818289110817
128652_at 4.43270483847079

Total number of rows: 11934

Table truncated, full table size 322 Kbytes.




Supplementary file Size Download File type/resource
GSM2732503_Norma0528BP1a_C.CEL.gz 2.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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