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Sample GSM2732505 Query DataSets for GSM2732505
Status Public on Aug 05, 2017
Title Liver_Human_Transgenic_SREBP2_chipC_rep2
Sample type RNA
 
Source name Liver, transgenic SREBP-2 mouse
Organism Mus musculus
Characteristics genotype: TgSREBP-2
age: 12-16 weeks
Sex: Male
diet: high protein/low carbohydrate
tissue: Liver
exposure time: 2 weeks
Treatment protocol Livers were excised for RNA extraction
Growth protocol Mice were housed in colony cages in rooms maintained on a 12-h light/12-h dark cycle.
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from each liver by using RNA STAT-60 (Tel-Test, Friendswood, TX)
Label biotin
Label protocol Equal aliquots of total RNA from each of five mouse livers in each group were pooled (total, 20 μg) and used for biotin labeling as described in the Affymetrix technical bulletin
 
Hybridization protocol Hybridization and washing of the Affymetrix GeneChip Murine Genome MU74A, -B, and -C version 2 arrays (Affymetrix, Santa Clara, CA) were carried out as described in: Mahadevappa, M. & Warrington, J. A. (1999) Nat. Biotechnol. 17, 1134–1136. Duplicate hybridizations were performed with each RNA sample.
Scan protocol Scanning, and analysis of the Affymetrix GeneChip Murine Genome MU74A, -B, and -C version 2 arrays (Affymetrix, Santa Clara, CA) were carried out as described in: Mahadevappa, M. & Warrington, J. A. (1999) Nat. Biotechnol. 17, 1134–1136.
Description Mice were fed a high protein/low carbohydrate diet for 2 weeks. This diet was used to elicit high-level expression of the nSREBPs
Data processing Gene expression results were analyzed by standard methods using Robust Multichip Average (RMA) normalization.
 
Submission date Aug 04, 2017
Last update date Jan 23, 2018
Contact name Christopher Corton
E-mail(s) corton.chris@epa.gov
Organization name US-EPA
Lab MD B105-01
Street address 109 TW Alexander Dr.
City Research Triangle Park
State/province NC
ZIP/Postal code 27711
Country USA
 
Platform ID GPL83
Series (2)
GSE102258 Combined analysis of oligonucleotide microarray data from transgenic and knockout mice identifies direct SREBP target genes [MG_U74Cv2]
GSE102259 Combined analysis of oligonucleotide microarray data from transgenic and knockout mice identifies direct SREBP target genes

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
128520_at 9.28359763928045
128523_at 2.99213987157906
128529_at 4.44444026606839
128550_at 5.63390284883922
128554_at 5.10738932081616
128577_s_at 4.34919589124764
128578_r_at 3.60481045951887
128579_r_at 5.04529298251338
128580_at 5.76361911268849
128582_at 4.78783300177244
128583_at 3.99273119847532
128590_at 4.88807573731456
128624_at 3.91985510596625
128643_r_at 7.65828684756304
128644_at 7.10506098113363
128645_at 5.37095985626109
128646_at 4.64957321458418
128647_at 6.47703885946884
128651_at 7.06680491510944
128652_at 4.57719129139112

Total number of rows: 11934

Table truncated, full table size 322 Kbytes.




Supplementary file Size Download File type/resource
GSM2732505_Norma0528BP2_C.CEL.gz 2.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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