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Sample GSM2735427 Query DataSets for GSM2735427
Status Public on Jun 01, 2018
Title Venous blood_d34_ctrl_4515
Sample type RNA
 
Source name male_control_whole blood
Organism Gallus gallus
Characteristics chicken id: chicken 4515
gender: male
age: 34 days after birth
sample group: control
tissue: whole, venous blood
Treatment protocol For 24h after leaving the hatchery, the chicks were kept without food and water in containers and subjected to a lower room temperature (22°C = cold vs 27°C = control) and shaking movements mimicking transport. Control animals were not be subjected to stressors after leaving the hatchery and were placed in standard rearing conditions. Venous blood was collected from the 24 chicks 34 days after laying, using vacuum tubes with EDTA. 100µl of whole blood were diluted in 1ml of trizol. After a strong shaking and 5 mn of incubation at RT, the tubes were frozen in liquid nitrogen and stored at -80°C until extraction.
Extracted molecule total RNA
Extraction protocol Before extraction, 10µl of acetic acid 5N was added in each tube. Total RNA was extracted with TRIzol reagent (Invitrogen) according to the manufacturer’s protocol.
Label Cy3
Label protocol Cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent) according to the manufacturer's instructions, followed by Agencourt RNAClean XP (Agencourt Bioscience Corporation, Beverly, Massachusetts). Dye incorporation and cRNA yield were checked using Dropsense™ 96 UV/VIS droplet reader (Trinean, Belgium).
 
Hybridization protocol 600 ng of Cy3-labelled cRNA were hybridized on the microarray slides following the manufacturer’s instructions.
Scan protocol Immediately after washing, the slides were scanned on Agilent G2505C Microarray Scanner using Agilent Scan Control A.8.5.1 software.
Description SAMPLE 3
Data processing The fluorescence signal extracted using Agilent Feature Extraction software v10.10.1.1 with default parameters.
Raw data (median signal intensity) were filtered, log2 transformed, and normalized using quantile method.
 
Submission date Aug 08, 2017
Last update date Jun 01, 2018
Contact name Aline FOURY
E-mail(s) aline.foury@inrae.fr
Organization name INRAE
Lab NutriNeuro
Street address 146 rue Léo Saignat
City Bordeaux
ZIP/Postal code 33600
Country France
 
Platform ID GPL19630
Series (1)
GSE102358 Spontaneous intake of essential oils after a negative postnatal experience has long-term effects on blood transcriptome in chickens

Data table header descriptions
ID_REF
VALUE log2 normalized

Data table
ID_REF VALUE
5 7.997298547
8 12.43352314
9 8.184303434
10 13.3859879
11 8.245001624
12 6.856588831
13 9.555572131
14 11.96986075
16 9.60685826
17 7.401000163
18 10.71602849
19 10.52161264
20 5.127551386
21 7.33810971
22 6.017278107
23 5.484456597
24 5.289543403
25 5.876784949
26 10.63834263
27 5.60684646

Total number of rows: 52892

Table truncated, full table size 915 Kbytes.




Supplementary file Size Download File type/resource
GSM2735427_US10463851_254200410028_S01_GE1_1010_Sep10_2_2.txt.gz 11.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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