|
| Status |
Public on Feb 06, 2018 |
| Title |
CTL_2 [miRNome] |
| Sample type |
RNA |
| |
|
| Source name |
Kidney, control
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: CD-1
tissue: Kidney (total)
treatment: Physiological serum
amount in (ug): 0.1
rin number: 9
|
| Treatment protocol |
CD-1 mice were treated with Tacrolimus (1mg/kg/d for 28 days) or physiological serum as control.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted from kidneys using miRNeasy Mini kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
Agilent - Low RNA input QuickAmp.
|
| |
|
| Hybridization protocol |
Agilent : 100.0 ng at 55 degree_C during 20 hours.
|
| Scan protocol |
Arrays were scanned using the Agilent SureScan High Resolution Scanner.
|
| Description |
CTL_2
RNA extracted from control mouse kidneys (Replicate 2).
|
| Data processing |
The R package AgiMicroRna was used to extract the TotalGenesignal value from the Agilent result files. Quantile normalization and differential analysis were performed using Limma package in R / Bioconductor.
|
| |
|
| Submission date |
Aug 10, 2017 |
| Last update date |
Feb 07, 2018 |
| Contact name |
Kevin Lebrigand |
| Organization name |
IPMC/CNRS
|
| Lab |
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
|
| Street address |
660 route des lucioles
|
| City |
Valbonne - Sophia-Antipolis |
| ZIP/Postal code |
06560 |
| Country |
France |
| |
|
| Platform ID |
GPL23897 |
| Series (2) |
| GSE102514 |
Tacrolimus-induced nephrotoxicity in mice is associated with microRNA deregulation (miRNome analysis) |
| GSE102515 |
Tacrolimus-induced nephrotoxicity in mice is associated with microRNA deregulation |
|
