|
| Status |
Public on Dec 30, 2023 |
| Title |
US-1456777_16-VIS04-D31_L8.D704 |
| Sample type |
SRA |
| |
|
| Source name |
PBMC
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: PBMC
subject id: 16
time point: D31
timepoint_description: One day after 2nd vaccination
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Paxgene RNA
GlobinClear Globin transcript reduction followed by Illumina mRNA-Seq; 51bp PE
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Alignment STAR version STAR_2.3.1d
Sequenced reads were filtered for low-quality sequence and the presence of 'N's, then mapped to hg19 whole genome using star STAR_2.3.1d
Gene counts were calculated by using HTSeq count
Genome_build: hg19
Supplementary_files_format_and_content: tab delimited file including the raw read counts for all the detected genes
|
| |
|
| Submission date |
Aug 11, 2017 |
| Last update date |
Dec 30, 2023 |
| Contact name |
Daniel Zak |
| E-mail(s) |
danielzak000@gmail.com
|
| Organization name |
2Seventy Bio
|
| Lab |
Data Sciences
|
| Street address |
188 East Blaine Street, Suite 300
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98102 |
| Country |
USA |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE102574 |
RNA-Seq analysis of the TB-019 GSK M72/AS01E trial |
|
| Relations |
| BioSample |
SAMN07500852 |
| SRA |
SRX3089751 |
