NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM2748020 Query DataSets for GSM2748020
Status Public on Mar 01, 2018
Title CKCS_Saliva_Unaffected_2
Sample type genomic
 
Source name Saliva
Organism Canis lupus familiaris
Characteristics breed: Cavalier King Charles Spaniel
disease state: control
sm: 0
ccd size: 0
vd: 1
f-diam: 48.08
ab: 13.85
bc: 17.77
cd: 16.04
bd: 29.5
ae: 33.07
ID: 30.12
ac: 28.83
ai: 30.15
cj: 11.15
fh: 29.55
fg: 0
bk: 12.73
l1 (hae): 118.92
l2 (fac): 79.52
l3 (dib): 71.34
l4 (fae): 46.28
l5 (aeb): 24.66
l6(dba): 102.01
l7(dbi): 31.42
l8 (ebd): 8.39
l9 (jcb): 47.03
l10 (afg): 0
l11(ebk): 55.61
f-d/cd: 2.997506234
f-d/bc: 2.705683737
l4+l7: 77.7
Extracted molecule genomic DNA
Extraction protocol DNA was extracted from blood or saliva using the ORAGENE Animal kit (DNAgenotek)
Label CY3 and CY5
Label protocol 200ng of genomic DNA was whole-genome amplified in an overnight reaction at 37¡C using amplification master mix (WG-MA1, WG-MA2) and primer/neutralization mix (WG-MSM). After incubation the amplified DNA was fragmented with fragmentation mix (WG-FMS), precipitated with isopropanol and precipitation mix (PA1) and resuspended in hybridization buffer (RA1).
 
Hybridization protocol RA1 resuspended DNA was loaded onto BeadChips arrays. After overnight incubation at 48¡C, single-base extension and allele-specific staining was performed on a Teflow chamber rack system (Tecan, Maennedorf, Switzerland).
Scan protocol After allele-specific staining BeadChip arrays were coated with XC4/ethanol , dried for 1 hour and scanned on a iScan Autoloader 2X (Illumina).
Description 11404-D0002318-WG3318316-DNA-A02
Data processing Genomic DNA extracted from saliva was genotyped using Infinium CanineHD Genotyping BeadChips (Illumina).
GenomeStudio 2.0 was used to generate processed data using the final report function.
 
Submission date Aug 22, 2017
Last update date Mar 01, 2018
Contact name Philippe Lemay
E-mail(s) lemayph@gmail.com
Organization name Université de Montréal
Street address 3175 Chemin de la Côte-Sainte-Catherine
City Montreal
State/province Quebec
ZIP/Postal code H3T 1C5
Country Canada
 
Platform ID GPL17481
Series (1)
GSE102906 Whole genome QTL study identifies candidate loci associated to syringomyelia

Data table header descriptions
ID_REF
VALUE Genotype: AA,AB,BB,or NC (no call)
GC_SCORE
Theta
R
B_Allele_Freq
Log_R_Ratio

Data table
ID_REF VALUE GC_SCORE Theta R B_Allele_Freq Log_R_Ratio
BICF2G630100019 AA 0.9546 0.009 0.939 4,00E-04 0.1201
BICF2G630100032 AA 0.951 0.01 1.029 0 0.0514
BICF2G630100034 BB 0.9357 0.977 1.153 0.992 -1,00E-04
BICF2G630100043 AA 0.8037 0.019 1.061 2,00E-04 0.1297
BICF2G630100054 AB 0.9124 0.422 0.397 0.5329 -0.5281
BICF2G630100063 AB 0.8383 0.59 1.614 0.5797 -0.1004
BICF2G630100075 AA 0.9335 0.036 0.622 0.0084 0.0435
BICF2G63010009 BB 0.8763 0.966 0.789 0.9965 -0.2922
BICF2G630100090 BB 0.9083 0.983 1.119 0.996 0.0906
BICF2G630100094 BB 0.9 0.998 1.401 0.9994 0.0334
BICF2G63010010 BB 0.8247 0.943 0.739 0.9595 -0.2796
BICF2G630100114 BB 0.9349 0.982 1.145 1 0.0393
BICF2G630100129 AA 0.8318 0.024 1.414 0 0.0334
BICF2G63010014 NC 0 0.687 0.592 0.6362 -0.17
BICF2G630100157 AB 0.868 0.482 1.518 0.5363 -0.0665
BICF2G63010018 AA 0.3233 0.086 0.304 0.0314 -1.7543
BICF2G63010019 AA 0.8066 0.049 0.832 0 0.0131
BICF2G630100197 BB 0.5555 0.967 2.253 1 0.305
BICF2G630100209 AA 0.956 0.015 1.265 0 0.0772
BICF2G630100251 AA 0.9101 0.022 1.299 0.0036 0.0745

Total number of rows: 173662

Table truncated, full table size 8314 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap