|
Status |
Public on Mar 01, 2018 |
Title |
CKCS_Saliva_Unaffected_17 |
Sample type |
genomic |
|
|
Source name |
Saliva
|
Organism |
Canis lupus familiaris |
Characteristics |
breed: Cavalier King Charles Spaniel disease state: control sm: 0 ccd size: 0 vd: 2 f-diam: 44.17 ab: 14.69 bc: 16.79 cd: 12.7 bd: 27.67 ae: 32.44 ID: 29.22 ac: 27.36 ai: 26.52 cj: 12.5 fh: 24.89 fg: 0 bk: 11.01 l1 (hae): 119.99 l2 (fac): 69.54 l3 (dib): 74.89 l4 (fae): 42.56 l5 (aeb): 27.16 l6(dba): 98.97 l7(dbi): 23.81 l8 (ebd): 7.06 l9 (jcb): 43.35 l10 (afg): 0 l11(ebk): 48.16 f-d/cd: 3.477952756 f-d/bc: 2.630732579 l4+l7: 66.37
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA was extracted from blood or saliva using the ORAGENE Animal kit (DNAgenotek)
|
Label |
CY3 and CY5
|
Label protocol |
200ng of genomic DNA was whole-genome amplified in an overnight reaction at 37¡C using amplification master mix (WG-MA1, WG-MA2) and primer/neutralization mix (WG-MSM). After incubation the amplified DNA was fragmented with fragmentation mix (WG-FMS), precipitated with isopropanol and precipitation mix (PA1) and resuspended in hybridization buffer (RA1).
|
|
|
Hybridization protocol |
RA1 resuspended DNA was loaded onto BeadChips arrays. After overnight incubation at 48¡C, single-base extension and allele-specific staining was performed on a Teflow chamber rack system (Tecan, Maennedorf, Switzerland).
|
Scan protocol |
After allele-specific staining BeadChip arrays were coated with XC4/ethanol , dried for 1 hour and scanned on a iScan Autoloader 2X (Illumina).
|
Description |
11404-D0002884-WG3318316-DNA-B05
|
Data processing |
Genomic DNA extracted from saliva was genotyped using Infinium CanineHD Genotyping BeadChips (Illumina). GenomeStudio 2.0 was used to generate processed data using the final report function.
|
|
|
Submission date |
Aug 22, 2017 |
Last update date |
Mar 01, 2018 |
Contact name |
Philippe Lemay |
E-mail(s) |
lemayph@gmail.com
|
Organization name |
Université de Montréal
|
Street address |
3175 Chemin de la Côte-Sainte-Catherine
|
City |
Montreal |
State/province |
Quebec |
ZIP/Postal code |
H3T 1C5 |
Country |
Canada |
|
|
Platform ID |
GPL17481 |
Series (1) |
GSE102906 |
Whole genome QTL study identifies candidate loci associated to syringomyelia |
|