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Sample GSM2754480 Query DataSets for GSM2754480
Status Public on Dec 19, 2019
Title CL3
Sample type SRA
Source name MA9.3RAS
Organism Homo sapiens
Characteristics tumor: acute myeloid leukemia
treatment: HHT 10ng/ml
Extracted molecule polyA RNA
Extraction protocol RNA was extracted by using TRIzol reagent (Invitrogen, Carlsbad, CA). NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs, Ipswich, MA) was used for polyA RNA purification.
Library was prepared by or NEBNext Ultra Directional RNA Library Prep Kit (New England BioLabs, Ipswich, MA).
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1000
Data processing sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence
the RPKM were calculated using RSEM 1.2.31 with default parameters
Genome_build: GRCh38
Supplementary_files_format_and_content: .xls file containing the expression and p value
Submission date Aug 25, 2017
Last update date Dec 19, 2019
Contact name Jianjun Chen
Phone 6262185591
Organization name Beckman Research Institute of City of Hope
Department Systems Biology
Lab Chen Lab
Street address 1218 S. 5th Ave., Room 2062
City Monrovia
State/province CA
ZIP/Postal code 91016
Country USA
Platform ID GPL15433
Series (1)
GSE103143 The mechanism of HHT in treating acute myeloid leukemia on RNA level.
BioSample SAMN07562179
SRA SRX3135953

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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