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Sample GSM2756344 Query DataSets for GSM2756344
Status Public on Feb 16, 2018
Title OUH_miR_067
Sample type RNA
 
Channel 1
Source name Primary breast tumour of patient with relapse
Organism Homo sapiens
Characteristics tissue: primary breast tumour
gender: female
pair no.: 34
metastasis (1= yes; 0=no): 1
tumour type: ILC
esr1 expression (1=yes; 0=no): 1
lymph node status (1=positive; 0=negative): 0
status at last follow up (1=dead, 0=alive): 1
Treatment protocol After surgical removal, tumor samples were snap-frozen and stored at -80°C.
Growth protocol not applicable
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from tumor samples with Trizol (Invitrogen) and further purified with the RNeasy micro kit (Qiagen), including DNase treatment. NanoDrop Spectrophotometer (NanoDrop Technologies) was used for RNA quantification. The quality of extracted RNA was assessed with Bioanalyzer 2100 (Agilent Technologies) using the RNA 6000 Nano Kit (Agilent Technologies).
Label Hy3
Label protocol The miRCURY Power labeling kit (Exiqon), miRCURY LNA Array hybridization buffer (Exiqon) and miRCURY LNA Array Washing buffer kit (Exiqon) were used for sample labelling, hybridization and washing, respectively.
 
Channel 2
Source name common reference pool of all samples
Organism Homo sapiens
Characteristics tissue: primary breast tumour
gender: female
Treatment protocol After surgical removal, tumor samples were snap-frozen and stored at -80°C.
Growth protocol not applicable
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from tumor samples with Trizol (Invitrogen) and further purified with the RNeasy micro kit (Qiagen), including DNase treatment. NanoDrop Spectrophotometer (NanoDrop Technologies) was used for RNA quantification. The quality of extracted RNA was assessed with Bioanalyzer 2100 (Agilent Technologies) using the RNA 6000 Nano Kit (Agilent Technologies).
Label Hy5
Label protocol The miRCURY Power labeling kit (Exiqon), miRCURY LNA Array hybridization buffer (Exiqon) and miRCURY LNA Array Washing buffer kit (Exiqon) were used for sample labelling, hybridization and washing, respectively.
 
 
Hybridization protocol Hybridization, washing, and scanning (Agilent G2565CA Microarray scanner) were performed according to the recommendations provided by Exiqon.
Scan protocol Scanned images were imported into GenePixPro6.0 software (Molecular Devices) for quality control and raw data extraction.
Description Total RNA extracted from fresh frozen primary breast tumour of a female patient with relapse
Data processing Signals from 3 spots were compiled, only human microRNAs were selected, the R-package limma was used for LOESS normalization and quantile normalization of raw signal intensities, and the ComBat function embedded in the sva R-package was used for adjustment of the normalized intensities and to eliminate potential batch effects
 
Submission date Aug 28, 2017
Last update date Jan 29, 2019
Contact name Ines Block
Organization name Odense University Hospital
Department Department of Clinical Genetics
Street address J.B. Winsløws Vej 4
City Odense
ZIP/Postal code 5000
Country Denmark
 
Platform ID GPL23960
Series (1)
GSE103161 MicroRNA profiles of 110 primary breast cancer tumors of systemically untreated, lymph node negative and estrogen receptor positive patients

Data table header descriptions
ID_REF
VALUE normalized log2 ratio between Hy3 (individual samples) and Hy5 (common references);

Data table
ID_REF VALUE
17888 -0.074240472
147162 -0.278403596
42530 -2.546876723
42769 0.454130932
147165 -0.489434656
42653 -0.438397681
145820 -0.945916039
145633 -0.152414511
145968 -1.570288053
42743 -0.857608271
145846 -0.250280839
17752 -0.245477248
145840 -0.322510021
42742 -3.316052445
42778 0.237756008
46438 -2.686193119
145746 -1.122400092
9938 -0.771375683
10916 -0.650582068
145694 0.080027747

Total number of rows: 1296

Table truncated, full table size 23 Kbytes.




Supplementary file Size Download File type/resource
GSM2756344_OUH_miR_067.gpr.gz 716.4 Kb (ftp)(http) GPR
Processed data included within Sample table

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