|
| Status |
Public on Jul 09, 2019 |
| Title |
Wild type, Rep#2 |
| Sample type |
RNA |
| |
|
| Source name |
embryonic stomach
|
| Organism |
Mus musculus |
| Characteristics |
developmental stage: E13.5 embryo
tissue: stomach
genotype: wild type
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
RMA method in Affy package from Bioconductor was used to summarize the probe level data and normalize the dataset to remove across array variation.
|
| |
|
| Submission date |
Aug 29, 2017 |
| Last update date |
Jul 09, 2019 |
| Contact name |
Julie Zhu |
| E-mail(s) |
julie.zhu@umassmed.edu
|
| Phone |
5088565377
|
| Organization name |
UMass Medical School
|
| Department |
Molecular Cellular and Cancer Biology
|
| Street address |
364 Plantation Street
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE103235 |
Gene expression analysis of wild type and Hedgehog pathway-activated embryonic mouse stomach |
|
