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Sample GSM2758656 Query DataSets for GSM2758656
Status Public on Jul 09, 2019
Title Wild type, Rep#3
Sample type RNA
 
Source name embryonic stomach
Organism Mus musculus
Characteristics developmental stage: E13.5 embryo
tissue: stomach
genotype: wild type
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Data processing RMA method in Affy package from Bioconductor was used to summarize the probe level data and normalize the dataset to remove across array variation.
 
Submission date Aug 29, 2017
Last update date Jul 09, 2019
Contact name Julie Zhu
E-mail(s) julie.zhu@umassmed.edu
Phone 5088565377
Organization name UMass Medical School
Department Molecular Cellular and Cancer Biology
Street address 364 Plantation Street
City Worcester
State/province MA
ZIP/Postal code 01605
Country USA
 
Platform ID GPL6246
Series (1)
GSE103235 Gene expression analysis of wild type and Hedgehog pathway-activated embryonic mouse stomach

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
10338001 12.5065128
10338002 8.818734799
10338003 10.90348683
10338004 9.659363196
10338005 4.140366681
10338006 4.52453267
10338007 5.228659118
10338008 6.424076774
10338009 10.25045305
10338010 4.166549212
10338011 8.413037331
10338012 4.329606476
10338013 3.983865239
10338014 4.042477605
10338015 4.005818118
10338016 9.898859829
10338017 13.13869514
10338018 9.279897871
10338019 7.978292661
10338020 10.28796804

Total number of rows: 35556

Table truncated, full table size 725 Kbytes.




Supplementary file Size Download File type/resource
GSM2758656_3_MoGene-1_0-st-v1.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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