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Sample GSM2779590 Query DataSets for GSM2779590
Status Public on Sep 12, 2017
Title Y1.7-P-Cd45neg-1
Sample type SRA
 
Source name CD45- fraction of YUMM1.7 melanoma
Organism Mus musculus
Characteristics tissue: YUMM1.7 melanoma tumor
mapki treatment: none
mapki type: none
Extracted molecule polyA RNA
Extraction protocol QIAGEN RNeasy micro kit
TruSeq RNA Sample Preparation Kit v2, unstranded
Poly A transcript enrichment, paired end RNAseq
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
 
Description Sorted CD45- cell fraction of YUMM1.7 melanoma tumor, pre BRAFi treatment, 1st tumor
Data processing FASTQ files were mapped by Tophat2
Tophat BAMs were quantified and normalized using Cuffnorm, DGE were called by cuffdiff
Genome_build: mm9
Supplementary_files_format_and_content: The normalized expression levels in FPKM called by cuffnorm
 
Submission date Sep 11, 2017
Last update date May 15, 2019
Contact name Willy Hugo
E-mail(s) hwilly@mednet.ucla.edu
Organization name UCLA
Department Medicine
Street address 10833 Le Conte Ave, 52-121 CHS, Division of Dermatology
City Los Angeles
State/province California
ZIP/Postal code 90095
Country USA
 
Platform ID GPL21493
Series (1)
GSE103712 Expression changes in CD45neg subpopulation of YUMM1.7 melanoma pre MAPKi treatment vs. on MAPKi treatment [Y1.7.FPKM.batch3]
Relations
BioSample SAMN07629757
SRA SRX3176871

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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