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Sample GSM2793818 Query DataSets for GSM2793818
Status Public on Feb 10, 2021
Title Kdm4b_KO_OI
Sample type SRA
 
Source name mMSCs from bone marrow
Organism Mus musculus
Characteristics cell type: mesenchymal stem cell (MSC)
tissue: bone marrow
genotype/variation: KDM4B KO
condition: osteogenic induction
Growth protocol Primary mBMSCs cultured in presence or absence of osteogenic induction for 4 days.
Extracted molecule polyA RNA
Extraction protocol Total RNA was isolated, and purified with Dynabeads™ mRNA Purification Kit (Invitrogen, Cat No: 61006).
Libaray was prepared using Stranded RNA-Seq Library Preparation Kit (Kapa Biosystems, Cat No: KK8400)
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
 
Description Kdm4b_KO_OI
Data processing The reads were aligned to the mm9 build of the mouse genome using Tophat.
Expression of the transcript was determined using Cufflinks with Refseq mRNAs to guide assembly.
Genome_build: mm9
Supplementary_files_format_and_content: Excel file with FPKM
 
Submission date Sep 26, 2017
Last update date Feb 10, 2021
Contact name Cunyu Wang
E-mail(s) cwang@dentistry.ucla.edu
Phone 3108256317
Organization name UCLA
Department School of Dentistry
Lab CHS 56-200
Street address 10833 Le Conte Ave
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platform ID GPL21493
Series (2)
GSE104258 Loss of KDM4B Exacerbates Bone-Fat Imbalance and Mesenchymal Stem Cell Exhaustion in Skeletal Aging [RNA-seq]
GSE104259 Loss of KDM4B exacerbates bone-fat imbalance and mesenchymal stromal cell exhaustion in skeletal aging
Relations
BioSample SAMN07702312
SRA SRX3216819

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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