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Sample GSM2793962 Query DataSets for GSM2793962
Status Public on Oct 19, 2017
Title 143B_Cybrid C/143BRho0
Sample type RNA
 
Channel 1
Source name 143B Osteosarcoma Parental Cells with depleted mitochondrial DNA fused with platelet cells from human origin
Organism Homo sapiens
Characteristics tissue: 143B Osteosarcoma Parental Cells with depleted mitochondrial DNA fused with platelet cells from human origin
Extracted molecule total RNA
Extraction protocol RNA was isolated using Trizol according to manufacturer's protocol.
Label Hy5
Label protocol Labelling was done with the miRCURY LNA array power labeling kit. The protocol was conducted by a commerical company according to proprietary protocols.
 
Channel 2
Source name 143B Osteosarcoma Parental Cells with depleted mitochondrial DNA
Organism Homo sapiens
Characteristics tissue: 143B Osteosarcoma Parental Cells with depleted mitochondrial DNA
Extracted molecule total RNA
Extraction protocol RNA was isolated using Trizol according to manufacturer's protocol.
Label Hy3
Label protocol Labelling was done with the miRCURY LNA array power labeling kit. The protocol was conducted by a commerical company according to proprietary protocols.
 
 
Hybridization protocol The array and all of its hybridization, washing etc was conducted by a commercial company according to their in house protocols.
Scan protocol The array scanning and image acquisition were conducted by a commercial company according to their in house protocols.
Data processing Data were normalized using the non-linear regression method (LOWESS)
 
Submission date Sep 26, 2017
Last update date Jan 23, 2018
Contact name Keshav Singh
E-mail(s) kksingh@uab.edu
Organization name University of Alabama at Birmingham
Department Genetics
Street address 720 20th Street South Kaul 630
City Birmingham
State/province AL
ZIP/Postal code 35294
Country USA
 
Platform ID GPL7724
Series (1)
GSE104277 Control of microRNA expression by mitochondrial function

Data table header descriptions
ID_REF
VALUE LOWESS normalized Logmedian ratios (sample/143B rho0)

Data table
ID_REF VALUE
17748 -0.11
17749 0.51
19004 0.33
17750
17751 0.24
17752
19602
19580 -0.63
19581 -0.29
31026 -0.25
10919 -0.01
28737 0.05
19582 -0.14
17854 -0.05
28481 0.03
13485 -0.11
10925 -0.43
10928 -0.07
30787 -0.27
10934

Total number of rows: 180

Table truncated, full table size 1 Kbytes.




Supplementary file Size Download File type/resource
GSM2793962_143BcybridC-143Brho0_Raw.xlsx 147.5 Kb (ftp)(http) XLSX
GSM2793962_slide_3_-_143B_Cybrid_C_Cy5.txt.gz 899.6 Kb (ftp)(http) TXT
GSM2793962_slide_3_-_143Brho0_Cy3.txt.gz 913.7 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record
Processed data provided as supplementary file

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