|
| Status |
Public on Sep 05, 2008 |
| Title |
SL2-GST-replicate1 |
| Sample type |
RNA |
| |
|
| Source name |
SL2 cell line
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cells treated with GST dsRNA
|
| Treatment protocol |
1x10e6 cells were incubated with 10 µg dsRNA in serum-free medium for 1 hours. Serum-containing medium was added and cells were harvested after 7 days without further passaging.
|
| Growth protocol |
Cells were grown in 6-well plates in Schneider Medium supplemented with 10% FCS, penicillin and streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen RNAEasy Mini kits including Qiagen DNAse on-column digestion according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
RNA labelling was performed by ImaGenes (http://www.imagenes-bio.de)
|
| |
|
| Hybridization protocol |
Hybridization was performed by ImaGenes (http://www.imagenes-bio.de)
|
| Scan protocol |
Scanning was performed by ImaGenes (http://www.imagenes-bio.de)
|
| Description |
GST1
|
| Data processing |
Raw data was processed using R/Bioconductor. Normalization and probeset expression values were calculated using GCRMA.
|
| |
|
| Submission date |
Apr 14, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Tobias Straub |
| E-mail(s) |
tstraub@med.uni-muenchen.de
|
| Organization name |
LMU Munich
|
| Department |
Biomedical Center, Bioinformatics
|
| Street address |
Großhadener Str. 9
|
| City |
Martinsried |
| ZIP/Postal code |
82152 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE11164 |
ISWI RNAi in Drosophila SL2 cells |
|
| Relations |
| Reanalyzed by |
GSE119084 |
