|
Status |
Public on Sep 05, 2008 |
Title |
SL2-ISWI-replicate2 |
Sample type |
RNA |
|
|
Source name |
SL2 cell line
|
Organism |
Drosophila melanogaster |
Characteristics |
cells treated with ISWI dsRNA
|
Treatment protocol |
1x10e6 cells were incubated with 10 µg dsRNA in serum-free medium for 1 hours. Serum-containing medium was added and cells were harvested after 7 days without further passaging.
|
Growth protocol |
Cells were grown in 6-well plates in Schneider Medium supplemented with 10% FCS, penicillin and streptomycin.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using Qiagen RNAEasy Mini kits including Qiagen DNAse on-column digestion according to manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
RNA labelling was performed by ImaGenes (http://www.imagenes-bio.de)
|
|
|
Hybridization protocol |
Hybridization was performed by ImaGenes (http://www.imagenes-bio.de)
|
Scan protocol |
Scanning was performed by ImaGenes (http://www.imagenes-bio.de)
|
Description |
ISWI2
|
Data processing |
Raw data was processed using R/Bioconductor. Normalization and probeset expression values were calculated using GCRMA.
|
|
|
Submission date |
Apr 14, 2008 |
Last update date |
Aug 28, 2018 |
Contact name |
Tobias Straub |
E-mail(s) |
tstraub@med.uni-muenchen.de
|
Organization name |
LMU Munich
|
Department |
Biomedical Center, Bioinformatics
|
Street address |
Großhadener Str. 9
|
City |
Martinsried |
ZIP/Postal code |
82152 |
Country |
Germany |
|
|
Platform ID |
GPL1322 |
Series (1) |
GSE11164 |
ISWI RNAi in Drosophila SL2 cells |
|
Relations |
Reanalyzed by |
GSE119084 |