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Sample GSM281630 Query DataSets for GSM281630
Status Public on Sep 05, 2008
Title SL2-ISWI-replicate2
Sample type RNA
 
Source name SL2 cell line
Organism Drosophila melanogaster
Characteristics cells treated with ISWI dsRNA
Treatment protocol 1x10e6 cells were incubated with 10 µg dsRNA in serum-free medium for 1 hours. Serum-containing medium was added and cells were harvested after 7 days without further passaging.
Growth protocol Cells were grown in 6-well plates in Schneider Medium supplemented with 10% FCS, penicillin and streptomycin.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Qiagen RNAEasy Mini kits including Qiagen DNAse on-column digestion according to manufacturer's instructions.
Label biotin
Label protocol RNA labelling was performed by ImaGenes (http://www.imagenes-bio.de)
 
Hybridization protocol Hybridization was performed by ImaGenes (http://www.imagenes-bio.de)
Scan protocol Scanning was performed by ImaGenes (http://www.imagenes-bio.de)
Description ISWI2
Data processing Raw data was processed using R/Bioconductor. Normalization and probeset expression values were calculated using GCRMA.
 
Submission date Apr 14, 2008
Last update date Aug 28, 2018
Contact name Tobias Straub
E-mail(s) tstraub@med.uni-muenchen.de
Organization name LMU Munich
Department Biomedical Center, Bioinformatics
Street address Großhadener Str. 9
City Martinsried
ZIP/Postal code 82152
Country Germany
 
Platform ID GPL1322
Series (1)
GSE11164 ISWI RNAi in Drosophila SL2 cells
Relations
Reanalyzed by GSE119084

Data table header descriptions
ID_REF
VALUE GCRMA expression value.

Data table
ID_REF VALUE
1616608_a_at 9.331036885
1622892_s_at 8.230299771
1622893_at 2.393258998
1622894_at 2.236825499
1622895_at 9.01722571
1622896_at 9.115718973
1622897_at 2.170458383
1622898_a_at 9.433138848
1622899_at 3.583659147
1622900_at 2.211525147
1622901_at 2.13382773
1622902_at 2.102273803
1622903_s_at 7.786286054
1622904_at 2.140524962
1622905_at 2.552650097
1622906_at 2.319227886
1622907_at 8.827496605
1622908_a_at 10.99312708
1622909_at 10.21642418
1622910_at 2.322605297

Total number of rows: 18952

Table truncated, full table size 432 Kbytes.




Supplementary file Size Download File type/resource
GSM281630.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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