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Sample GSM285668 Query DataSets for GSM285668
Status Public on Oct 04, 2008
Title Human dermal blood vascular endothelial cell 4
Sample type RNA
 
Source name Human primary dermal microvascular blood endothelial cell (BEC) isolated from neonatal human foreskin
Organism Homo sapiens
Characteristics CD45-/CD34+ Human Blood Vascular Endothelial Cell (BEC)
Growth protocol Cells were propagated in endothelial cell basal medium (Cambrex Corp.; East Rutherford, NJ ) containing 20% fetal bovine serum, antibiotics, 2 mmol/L L-glutamine (Invitrogen Corp.; Carlsbad, CA), 10 µg/mL hydrocortisone acetate and 2.5  10-2 mg/ml N-6,2’-O-dibutyryl adenosine-3,5’-cyclic monophosphate (Sigma-Aldrich; St. Louis, MO).
Extracted molecule total RNA
Extraction protocol Isolate total cellular RNA with 1 ml of the Trizol reagent (Invitrogen) after washing one 80-90 % confluent 10 cm culture dish with PBS. Homogenize the sample using a cell scraper and add 0.2 ml of chloroform in a 1.5 ml tube. Shake the sample vigorously for 15 seconds and allow standing for 5 minutes. Centrifuge the resulting mixture at 12,000 x g for 15 minutes at 4 degree Celsius. Transfer the aqueous phase to a fresh tube and add 0.5 ml of iso-propanol and mix. Allow the sample to stand for 5 minutes at room temperature and centrifuge at 12,000 x g for 10 minutes at 4 degrees Celsius. Remove the supernatant and wash the RNA pellet by adding 1 ml of 75% ethanol. Vortex the sample and then centrifuge at 12,000 x g for 5 minutes at 4 degrees Celsius. Remove the ethanol and briefly dry the RNA pellet for 5 minutes and add 50 micro-liter of RNAase/DNAase free water and mix by repeated pipetting until RNA pellet is fully dissolved.
Label TaqMan Probe
Label protocol TaqMan™Quantitative RT-PCR - FAM
 
Hybridization protocol TaqMan™Quantitative RT-PCR
Scan protocol TaqMan™ RT-PCR - Fluorescence
Description BEC4
Data processing Raw data were normalized to the cycle number (Ct) of beta-Actin expression
 
Submission date Apr 30, 2008
Last update date Jul 25, 2008
Contact name Jay Woo Shin
E-mail(s) jay.shin@gsc.riken.jp
Organization name ETH Zurich
Department Institute of Pharmaceutical Sciences
Lab Michael Detmar
Street address Wolfgang-Paulistrasse 10; HCI H394
City Zurich
State/province Zurich
ZIP/Postal code 8093
Country Switzerland
 
Platform ID GPL6802
Series (2)
GSE11306 Quantification of vascular lineage-specific differentiation (cell type comparison)
GSE11308 Quantification of vascular lineage-specific differentiation

Data table header descriptions
ID_REF
VALUE Cell cycle number (Ct); 41 = undetermined

Data table
ID_REF VALUE
1 6.7162705
2 8.2415335
3 14.3296095
4 9.2713605
5 8.7797165
6 3.6733305
7 7.8483165
8 6.0139755
9 -0.2859285
10 10.0565265
11 -10.8753505
12 10.0506765
13 3.6676505
14 5.7103175
15 0.1916445
16 8.2582005
17 13.3099865
18 8.2623465
19 5.2973125
20 4.9601805

Total number of rows: 192

Table truncated, full table size 2 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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