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Sample GSM285675 Query DataSets for GSM285675
Status Public on Oct 04, 2008
Title Human immortalized dermal microvascular endothelial cell line - 1
Sample type RNA
 
Source name Human immortalized dermal microvascular endothelial cell line
Organism Homo sapiens
Characteristics Human immortalized dermal microvascular endothelial cell line
Growth protocol Cells were propagated in endothelial cell basal medium (Cambrex Corp.; East Rutherford, NJ ) containing 20% fetal bovine serum, antibiotics, 2 mmol/L L-glutamine (Invitrogen Corp.; Carlsbad, CA), 10 µg/mL hydrocortisone acetate and 2.5  10-2 mg/ml N-6,2’-O-dibutyryl adenosine-3,5’-cyclic monophosphate (Sigma-Aldrich; St. Louis, MO).
Extracted molecule total RNA
Extraction protocol Isolate total cellular RNA with 1 ml of the Trizol reagent (Invitrogen) after washing one 80-90 % confluent 10 cm culture dish with PBS. Homogenize the sample using a cell scraper and add 0.2 ml of chloroform in a 1.5 ml tube. Shake the sample vigorously for 15 seconds and allow standing for 5 minutes. Centrifuge the resulting mixture at 12,000 x g for 15 minutes at 4 degree Celsius. Transfer the aqueous phase to a fresh tube and add 0.5 ml of iso-propanol and mix. Allow the sample to stand for 5 minutes at room temperature and centrifuge at 12,000 x g for 10 minutes at 4 degrees Celsius. Remove the supernatant and wash the RNA pellet by adding 1 ml of 75% ethanol. Vortex the sample and then centrifuge at 12,000 x g for 5 minutes at 4 degrees Celsius. Remove the ethanol and briefly dry the RNA pellet for 5 minutes and add 50 micro-liter of RNAase/DNAase free water and mix by repeated pipetting until RNA pellet is fully dissolved.
Label TaqMan Probe
Label protocol TaqMan™Quantitative RT-PCR - FAM
 
Hybridization protocol TaqMan™Quantitative RT-PCR
Scan protocol TaqMan™ RT-PCR - Fluorescence
Description HMEC-1
Data processing Raw data were normalized to the cycle number (Ct) of beta-Actin expression
 
Submission date Apr 30, 2008
Last update date Jul 25, 2008
Contact name Jay Woo Shin
E-mail(s) jay.shin@gsc.riken.jp
Organization name ETH Zurich
Department Institute of Pharmaceutical Sciences
Lab Michael Detmar
Street address Wolfgang-Paulistrasse 10; HCI H394
City Zurich
State/province Zurich
ZIP/Postal code 8093
Country Switzerland
 
Platform ID GPL6802
Series (2)
GSE11306 Quantification of vascular lineage-specific differentiation (cell type comparison)
GSE11308 Quantification of vascular lineage-specific differentiation

Data table header descriptions
ID_REF
VALUE Cell cycle number (Ct); 41 = undetermined

Data table
ID_REF VALUE
1 10.1130685
2 17.8389445
3 8.2695145
4 2.7098375
5 6.2343285
6 10.8155985
7 7.3409125
8 3.6597775
9 -0.4770345
10 6.8283915
11 -0.9445985
12 8.2384955
13 17.8389445
14 5.9662695
15 -1.1828715
16 11.1986145
17 17.8389445
18 9.7573675
19 2.8176905
20 0.8068785

Total number of rows: 192

Table truncated, full table size 2 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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