|
| Status |
Public on Nov 28, 2019 |
| Title |
Ind-NPSR-3 |
| Sample type |
SRA |
| |
|
| Source name |
induced nephron progenitor
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: induced nephron progenitor
culture media: NPSR
induced: doxycycline
clone: CRL1502 clone C32
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA were extracted using a Bioline Isolate II Mini kit
TruSeq stranded total RNA with RiboZero rRNA depletion
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Fastq files were generated using the bcl to fastQ converter 2.17.
The raw reads were quality and adapter trimmed using trimmomatic (v0.35)
The trimmed reads were mapped to the human genome (hg38) using the STAR aligner version 2.5.2a in the two pass mapping mode
The mapped reads were quantified at the gene level using featureCounts version 1.5.0-p3 using GENCODE Release 20 comprehensive annotation
Genome_build: hg38
Supplementary_files_format_and_content: Tab delimited text file with Gene id and Gene length in the first two columns, followed by 9 columns containing counts for each sample
|
| |
|
| Submission date |
Nov 28, 2017 |
| Last update date |
Nov 28, 2019 |
| Contact name |
Andrew Lonsdale |
| E-mail(s) |
andrew.lonsdale@mcri.edu.au
|
| Organization name |
Murdoch Children's Research Institute
|
| Street address |
50 Flemington Rd
|
| City |
Parkville |
| State/province |
VIC |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platform ID |
GPL18573 |
| Series (1) |
| GSE107410 |
Inducible three-factor direct reprogramming to nephron progenitors using piggyBac transposons |
|
| Relations |
| BioSample |
SAMN08103796 |
| SRA |
SRX3425796 |
