|
| Status |
Public on Jun 18, 2008 |
| Title |
primitive_hematopoietic_cells_enriched_for_multipotent_progenitors |
| Sample type |
SAGE |
| Anchor |
NlaIII
|
| Tag Count |
206613 |
| Tag Length |
17 |
| |
|
| Source name |
Human embryonic stem cell-derived primitive hematopoietic cells enriched for multipotent progenitors
|
| Organism |
Homo sapiens |
| Characteristics |
Cell line: Human embryonic stem cell line WA01 (H1)
Gender: Male
Karyotype: 46XY
Culture condition: hESC/OP9 coculture day 8 of differentiation
Estimated 19% CFU
Cell purification Method: Immuno-magnetic and Fluorescent Activated Cell Sorting (FACS)
Defining markers: Lin-CD34+CD43+CD45-
|
| Biomaterial provider |
Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison WI
|
| Growth protocol |
Growth medium: αMEM (Invitrogen) with 10% FBS (HyClone)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RiboPureTM Kit (Ambion, Austin, TX)
|
| |
|
| Description |
Assays to confirm viability: Colony Forming Cell Assay (CFCs)
No PCR amplification before SAGE
|
| |
|
| Submission date |
May 07, 2008 |
| Last update date |
Nov 19, 2008 |
| Contact name |
BCGSC BC Cancer Agency |
| E-mail(s) |
support@mouseatlas.org
|
| Organization name |
Canada's Michael Smith Genome Sciences Centre
|
| Department |
Gene Expression
|
| Lab |
Marco Marra
|
| Street address |
675 West 10th Avenue
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V5Z 1L3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1485 |
| Series (1) |
| GSE11795 |
Molecular Profiling of Primitive Hematopoietic Cells Generated In Vitro from Human Embryonic Stem Cells and In Vivo during Embryogenesis |
|
