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Sample GSM28837 Query DataSets for GSM28837
Status Public on Jun 13, 2005
Title ski2D_5
Sample type RNA
 
Channel 1
Source name BY4741 (wt) @ 26 deg
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name ski2D @ 26 deg
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description Reference and mutant strains grown at 26oC to an OD of 0.5-0.6 . RNA extracted.
 
Submission date Aug 12, 2004
Last update date Jun 13, 2005
Contact name Todd A Burckin
E-mail(s) burckin@biology.ucsc.edu
Phone 831 459-5245
Fax 831 459-3139
Organization name University of California Santa Cruz
Department Molecular, Cell, & Developmental Biology
Lab Hartzog Lab
Street address 1156 High Street
City Santa Cruz
State/province CA
ZIP/Postal code 95062
Country USA
 
Platform ID GPL1407
Series (1)
GSE1684 Exploring functional relationships between components of the gene expression machinery

Data table header descriptions
ID_REF
X X-coordinate of spot
Y Y-coordinate of spot
Dia. spot diameter in microns
F635 Median Channel 2 (Cy5/635) median pixel intensity - feature
F635 Mean Channel 2 (Cy5/635) mean pixel intensity - feature
F635 SD Channel 2 (Cy5/635) standard deviation of pixel intensities - feature
B635 Median Channel 2 (Cy5/635) median background intensity
B635 Mean Channel 2 (Cy5/635) mean background intensity
B635 SD Channel 2 (Cy5/635) standard deviation of background
% > B635+1SD Channel 2 (Cy5/635) percent of pixels 1 StDev above background
% > B635+2SD Channel 2 (Cy5/635) percent of pixels 2 StDevs above background
F635 % Sat. Channel 2 (Cy5/635) percent of pixels saturated
F532 Median Channel 1 (Cy3/532) median pixel intensity - feature
F532 Mean Channel 1 (Cy3/532) mean pixel intensity - feature
F532 SD Channel 1 (Cy3/532) standard deviation of pixel intensities - feature
B532 Median Channel 1 (Cy3/532) median background intensity
B532 Mean Channel 1 (Cy3/532) mean background intensity
B532 SD Channel 1 (Cy3/532) standard deviation of background
% > B532+1SD Channel 1 (Cy3/532) percent of pixels 1 StDev above background
% > B532+2SD Channel 1 (Cy3/532) percent of pixels 2 StDevs above background
F532 % Sat. Channel 1 (Cy3/532) percent of pixels saturated
Ratio of Medians Ratio of medians (Ch2/Ch1)
Ratio of Means Ratio of means (Ch2/Ch1)
Median of Ratios Median of ratios
Mean of Ratios Mean of ratios
Ratios SD Ratio standard deviation
Rgn Ratio Regression ratio
Rgn R? Regression square ratio
F Pixels Number of feature pixels
B Pixels Number of background pixels
Sum of Medians Sum of medians
Sum of Means Sum of means
VALUE Log ratio (base 2)
F635 Median - B635 Channel 2 (Cy5/635) background subtracted median feature intensity
F532 Median - B532 Channel 1 (Cy3/532) background subtracted median feature intensity
F635 Mean - B635 Channel 2 (Cy5/635) background subtracted mean feature intensity
F532 Mean - B532 Channel 1 (Cy3/532) background subtracted mean feature intensity
Flags User defined flag (default=0)

Data table
ID_REF X Y Dia. F635 Median F635 Mean F635 SD B635 Median B635 Mean B635 SD % > B635+1SD % > B635+2SD F635 % Sat. F532 Median F532 Mean F532 SD B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians Ratio of Means Median of Ratios Mean of Ratios Ratios SD Rgn Ratio Rgn R? F Pixels B Pixels Sum of Medians Sum of Means VALUE F635 Median - B635 F532 Median - B532 F635 Mean - B635 F532 Mean - B532 Flags
1 1990 23100 120 475 532 243 426 431 93 40 17 0 3362 3633 2268 412 416 98 97 97 0 0.017 0.033 0.062 0.354 0.764 0.005 0.004 120 584 2999 3327 -5.912 49 2950 106 3221 0
2 2140 23100 100 434 537 305 419 426 92 28 17 0 416 428 127 417 421 102 22 3 0 -15 10.727 0.868 2.841 4.381 0.426 0.062 80 353 14 129 15 -1 118 11 -50
3 2320 23100 100 444 480 159 415 421 98 33 12 0 441 453 114 410 417 104 25 10 0 0.935 1.512 1.635 4.835 14.544 0.114 0.011 80 417 60 108 -0.096 29 31 65 43 -50
4 2490 23110 100 425 505 260 407 421 108 22 13 0 411 428 139 405 417 107 20 6 0 3 4.261 1.027 3.156 6.035 0.379 0.065 80 464 24 121 1.585 18 6 98 23 -50
5 2680 23120 50 714 759 245 462 484 147 58 41 0 551 549 200 408 438 128 50 33 0 1.762 2.106 1.528 1.352 0.536 0.21 0.026 12 104 395 438 0.817 252 143 297 141 0
6 2870 23100 110 3137 3104 540 415 427 107 100 100 0 5617 5399 1186 402 418 111 100 100 0 0.522 0.538 0.545 0.566 0.155 0.513 0.956 80 523 7937 7686 -0.938 2722 5215 2689 4997 0
7 3040 23100 110 952 961 197 412 415 92 100 98 0 1438 1402 336 392 399 100 98 98 0 0.516 0.544 0.524 0.686 0.717 0.453 0.662 80 461 1586 1559 -0.954 540 1046 549 1010 0
8 3190 23110 100 458 513 247 414 414 90 37 20 0 421 447 128 395 401 103 28 11 0 1.692 1.904 1.466 3.494 4.216 0.42 0.098 80 424 70 151 0.759 44 26 99 52 -50
9 3400 23110 120 6802 6495 2870 405 416 128 99 99 0 4399 4257 1910 392 401 100 98 96 0 1.596 1.576 1.592 2.012 3.369 1.527 0.96 120 546 10404 9955 0.675 6397 4007 6090 3865 0
10 3570 23110 110 1567 1612 307 403 406 93 100 100 0 1109 1130 264 387 397 102 100 97 0 1.612 1.627 1.625 1.844 0.841 1.331 0.763 80 429 1886 1952 0.689 1164 722 1209 743 0
11 3710 23120 100 416 490 264 394 398 87 30 16 0 382 406 112 388 400 106 20 7 0 -3.667 5.333 1.407 2.303 2.52 0.555 0.112 80 393 16 114 22 -6 96 18 -50
12 3890 23120 100 430 474 224 406 407 88 26 11 0 377 390 112 382 394 105 17 6 0 -4.8 8.5 1.155 3.208 5.494 0.378 0.067 80 415 19 76 24 -5 68 8 -50
13 4060 23120 100 423 481 253 413 415 95 17 13 0 401 424 127 396 408 102 22 7 0 2 2.429 0.87 3.46 7.494 0.497 0.111 80 431 15 96 1 10 5 68 28 -50
14 4240 23120 100 427 489 235 407 414 98 33 15 0 411 416 116 401 405 96 20 7 0 2 5.467 1.163 5.34 15.435 0.337 0.047 80 488 30 97 1 20 10 82 15 -50
15 4470 23110 50 601 600 141 460 484 152 50 8 0 515 520 112 434 443 102 41 16 0 1.741 1.628 1.794 3.687 5.232 0.406 0.084 12 86 222 226 0.8 141 81 140 86 0
16 4590 23120 100 417 438 121 412 417 106 22 3 0 413 428 106 399 408 103 22 7 0 0.357 0.897 1.006 3.057 7.88 -0.02 0 80 428 19 55 -1.485 5 14 26 29 -50
17 4760 23120 100 398 415 120 393 413 125 15 3 0 419 425 103 375 386 103 31 5 0 0.114 0.44 0.817 1.327 1.839 0.13 0.015 80 441 49 72 -3.138 5 44 22 50 -50
18 4940 23130 100 444 463 140 406 419 116 17 10 0 396 395 95 395 397 99 15 3 0 38 100000 1.214 2.486 3.522 0.092 0.006 80 488 39 57 5.248 38 1 57 0 -50
19 5140 23100 70 609 651 222 414 422 115 65 40 0 642 674 131 430 449 142 78 34 0 0.92 0.971 0.936 1.259 1.136 0.436 0.184 32 197 407 481 -0.121 195 212 237 244 0
20 5290 23130 100 451 504 198 406 422 113 31 16 0 443 461 120 412 426 135 21 6 0 1.452 2 1.116 4.806 9.093 0.358 0.099 80 468 76 147 0.538 45 31 98 49 -50

Total number of rows: 5760

Table truncated, full table size 957 Kbytes.




Supplementary data files not provided

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