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Sample GSM2891007 Query DataSets for GSM2891007
Status Public on Dec 16, 2017
Title HBEC3_vector_miRNA
Sample type RNA
Source name HBEC3, empty retroviral vector
Organism Homo sapiens
Characteristics cell type: Human bronchial epithelial cells immortalized with hTERT and hCDK4
patient age: 65
patient gender: Female
patient smoking status: Smoker
patient diagnosis: No lung cancer as cells isolated from large airway
snail oe: no
Treatment protocol Transduced cells underwent two weeks of antibiotic selection (100ug/ml hygromycin). They were then passaged three times in plain growth medium and harvested upon reaching 75% confluency.
Growth protocol Grown in Keratinocyte medium supplemented with 30ug/ml Bovine Pituitary Extract (BPE) and 0.2ng/ml recombinant Epidermal Growth Factor (EGF), all from Invitrogen (#17005-042).
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Qiagen's Rneasy Mini Kit according to the manufacturer's instructions.
Label Hy3
Label protocol As recommended by manufacturer
Hybridization protocol As recommended by manufacturer. Arrays used are: miRCURY LNA microRNA Array, v.11.0 - hsa, mmu & rno
Scan protocol GenePix Personal 4100A Scanner and its software (GenePix Pro were used for scanning, and image processing.
Description Sample name: H3V1
Data processing Data was normalized by rmaMicroRna function in AgiMicroRna package (R/Bioconductor) which returned the data in log2 scale for individual probes
Submission date Dec 15, 2017
Last update date Dec 16, 2017
Contact name Linh My Tran
Organization name University of Los Angeles
Department Medicine - Pulmonary & Critical Care
Lab Dubinett Lab
Street address Box 951690, 37-131 CHS
City Los Angeles
State/province California
ZIP/Postal code 90095-1690
Country USA
Platform ID GPL7723
Series (2)
GSE108136 Snail-dependent epithelial splicing regulatory protein 1 (ESRP1) silencing drives malignant transformation of human pulmonary epithelial cells [miRNA]
GSE108137 Snail-dependent epithelial splicing regulatory protein 1 (ESRP1) silencing drives malignant transformation of human pulmonary epithelial cells

Data table header descriptions
VALUE log2 RMA signal

Data table
10618 8.191676146
10914 12.50711326
10962 7.717676423
1100 6.487840034
11089 6.599912842
11117 7.20701432
11135 8.64655871
11164 7.172427509
11176 7.250298418
11265 7.441906705
11275 7.864186145
13131 8.240195054
13141 8.178976011
13175 11.89111915
13186 5.95419631
13367 6.642051693
13371 6.861086906
13388 5.980139578
13389 6.878050913
13393 6.675957033

Total number of rows: 1996

Table truncated, full table size 34 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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