|
| Status |
Public on Dec 16, 2017 |
| Title |
HBEC4_vector_miRNA |
| Sample type |
RNA |
| |
|
| Source name |
HBEC4, empty retroviral vector
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Human bronchial epithelial cells immortalized with hTERT and hCDK4
patient age: 71
patient gender: Female
patient smoking status: Smoker
patient diagnosis: With lung cancer as cells isolated from large airway
snail oe: no
|
| Treatment protocol |
Transduced cells underwent two weeks of antibiotic selection (100ug/ml hygromycin). They were then passaged three times in plain growth medium and harvested upon reaching 75% confluency.
|
| Growth protocol |
Grown in Keratinocyte medium supplemented with 30ug/ml Bovine Pituitary Extract (BPE) and 0.2ng/ml recombinant Epidermal Growth Factor (EGF), all from Invitrogen (#17005-042).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen's Rneasy Mini Kit according to the manufacturer's instructions.
|
| Label |
Hy3
|
| Label protocol |
As recommended by manufacturer
|
| |
|
| Hybridization protocol |
As recommended by manufacturer. Arrays used are: miRCURY LNA microRNA Array, v.11.0 - hsa, mmu & rno
|
| Scan protocol |
GenePix Personal 4100A Scanner and its software (GenePix Pro 6.0.1.25) were used for scanning, and image processing.
|
| Description |
Sample name: H4V1
microRNA
|
| Data processing |
Data was normalized by rmaMicroRna function in AgiMicroRna package (R/Bioconductor) which returned the data in log2 scale for individual probes
|
| |
|
| Submission date |
Dec 15, 2017 |
| Last update date |
Dec 16, 2017 |
| Contact name |
Linh My Tran |
| E-mail(s) |
linhtran@ucla.edu
|
| Organization name |
University of Los Angeles
|
| Department |
Medicine - Pulmonary & Critical Care
|
| Lab |
Dubinett Lab
|
| Street address |
Box 951690, 37-131 CHS
|
| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90095-1690 |
| Country |
USA |
| |
|
| Platform ID |
GPL7723 |
| Series (2) |
| GSE108136 |
Snail-dependent epithelial splicing regulatory protein 1 (ESRP1) silencing drives malignant transformation of human pulmonary epithelial cells [miRNA] |
| GSE108137 |
Snail-dependent epithelial splicing regulatory protein 1 (ESRP1) silencing drives malignant transformation of human pulmonary epithelial cells |
|
