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Sample GSM2891009 Query DataSets for GSM2891009
Status Public on Dec 16, 2017
Title HBEC4_vector_miRNA
Sample type RNA
Source name HBEC4, empty retroviral vector
Organism Homo sapiens
Characteristics cell type: Human bronchial epithelial cells immortalized with hTERT and hCDK4
patient age: 71
patient gender: Female
patient smoking status: Smoker
patient diagnosis: With lung cancer as cells isolated from large airway
snail oe: no
Treatment protocol Transduced cells underwent two weeks of antibiotic selection (100ug/ml hygromycin). They were then passaged three times in plain growth medium and harvested upon reaching 75% confluency.
Growth protocol Grown in Keratinocyte medium supplemented with 30ug/ml Bovine Pituitary Extract (BPE) and 0.2ng/ml recombinant Epidermal Growth Factor (EGF), all from Invitrogen (#17005-042).
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Qiagen's Rneasy Mini Kit according to the manufacturer's instructions.
Label Hy3
Label protocol As recommended by manufacturer
Hybridization protocol As recommended by manufacturer. Arrays used are: miRCURY LNA microRNA Array, v.11.0 - hsa, mmu & rno
Scan protocol GenePix Personal 4100A Scanner and its software (GenePix Pro were used for scanning, and image processing.
Description Sample name: H4V1
Data processing Data was normalized by rmaMicroRna function in AgiMicroRna package (R/Bioconductor) which returned the data in log2 scale for individual probes
Submission date Dec 15, 2017
Last update date Dec 16, 2017
Contact name Linh My Tran
Organization name University of Los Angeles
Department Medicine - Pulmonary & Critical Care
Lab Dubinett Lab
Street address Box 951690, 37-131 CHS
City Los Angeles
State/province California
ZIP/Postal code 90095-1690
Country USA
Platform ID GPL7723
Series (2)
GSE108136 Snail-dependent epithelial splicing regulatory protein 1 (ESRP1) silencing drives malignant transformation of human pulmonary epithelial cells [miRNA]
GSE108137 Snail-dependent epithelial splicing regulatory protein 1 (ESRP1) silencing drives malignant transformation of human pulmonary epithelial cells

Data table header descriptions
VALUE log2 RMA signal

Data table
10618 8.208843991
10914 12.26885264
10962 7.540128039
1100 6.319672121
11089 6.857980995
11117 7.503825738
11135 8.005975357
11164 6.982993575
11176 7.189824559
11265 7.37286506
11275 7.977279923
13131 8.236014192
13141 8.176796478
13175 11.45269169
13186 6.335390355
13367 6.37286506
13371 6.731319031
13388 6.011227255
13389 7.081483438
13393 6.59058705

Total number of rows: 1996

Table truncated, full table size 34 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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