Data table header descriptions |
ID_REF |
|
X |
X-coordinate of spot |
Y |
Y-coordinate of spot |
Dia. |
spot diameter in microns |
F635 Median |
Channel 2 (Cy5/635) median pixel intensity - feature |
F635 Mean |
Channel 2 (Cy5/635) mean pixel intensity - feature |
F635 SD |
Channel 2 (Cy5/635) standard deviation of pixel intensities - feature |
B635 Median |
Channel 2 (Cy5/635) median background intensity |
B635 Mean |
Channel 2 (Cy5/635) mean background intensity |
B635 SD |
Channel 2 (Cy5/635) standard deviation of background |
% > B635+1SD |
Channel 2 (Cy5/635) percent of pixels 1 StDev above background |
% > B635+2SD |
Channel 2 (Cy5/635) percent of pixels 2 StDevs above background |
F635 % Sat. |
Channel 2 (Cy5/635) percent of pixels saturated |
F532 Median |
Channel 1 (Cy3/532) median pixel intensity - feature |
F532 Mean |
Channel 1 (Cy3/532) mean pixel intensity - feature |
F532 SD |
Channel 1 (Cy3/532) standard deviation of pixel intensities - feature |
B532 Median |
Channel 1 (Cy3/532) median background intensity |
B532 Mean |
Channel 1 (Cy3/532) mean background intensity |
B532 SD |
Channel 1 (Cy3/532) standard deviation of background |
% > B532+1SD |
Channel 1 (Cy3/532) percent of pixels 1 StDev above background |
% > B532+2SD |
Channel 1 (Cy3/532) percent of pixels 2 StDevs above background |
F532 % Sat. |
Channel 1 (Cy3/532) percent of pixels saturated |
Ratio of Medians (635/532) |
Ratio of medians (Ch2/Ch1) |
Ratio of Means (635/532) |
Ratio of means (Ch2/Ch1) |
Median of Ratios (635/532) |
Median of ratios |
Mean of Ratios (635/532) |
Mean of ratios |
Ratios SD (635/532) |
Ratio standard deviation |
Rgn Ratio (635/532) |
Regression ratio |
Rgn R? (635/532) |
Regression square ratio |
F Pixels |
Number of feature pixels |
B Pixels |
Number of background pixels |
Sum of Medians |
Sum of medians |
Sum of Means |
Sum of means |
VALUE |
Log ratio (base 2) |
F635 Median - B635 |
Channel 2 (Cy5/635) background subtracted median feature intensity |
F532 Median - B532 |
Channel 1 (Cy3/532) background subtracted median feature intensity |
F635 Mean - B635 |
Channel 2 (Cy5/635) background subtracted mean feature intensity |
F532 Mean - B532 |
Channel 1 (Cy3/532) background subtracted mean feature intensity |
F635 Total Intensity |
F635 Total Intensity |
F532 Total Intensity |
F532 Total Intensity |
SNR 635 |
n/a |
SNR 532 |
n/a |
Flags |
User defined flag (default=0) |
Normalize |
n/a |