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Sample GSM29080 Query DataSets for GSM29080
Status Public on Oct 18, 2004
Title SD5 - Replicate 1
Sample type RNA
 
Source name Total RNA
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Description Cells were grown to saturation in YPD (YEP + 2% glucose) for 24 hours, diluted into YPA (YEP + 2% potassium acetate) at OD600= 0.3 and grown over night at 30 degree C. Cells were washed with sterilized water the next day and re-suspended in SPO medium (0.3% potassium acetate, pH = 7.0) at OD600= 1.9 at room temperature to induce sporulation. DMSO (dimethyl sulfoxide, Sigma-Aldrich) was dissolved to a final concentration of 0.4% in near-boiling SPO medium. The medium was then allowed to slowly cool to room temperature. 4 h after induction of sporulation cells were filtered and immediately re-suspended in medium containing DMSO. The sample was taken 1 h after treatment with DMSO.
Keywords = benomyl sporulation saccharomyces microtubule spindle checkpoint
 
Submission date Aug 18, 2004
Last update date May 27, 2005
Contact name Michael Primig
E-mail(s) michael.primig@unibas.ch
Phone +41 61 267 2098
Fax +41 61 267 3398
URL http://www.biozentrum.unibas.ch/primig/
Organization name Biozentrum and Swiss Institute of Bioinformatics
Department Genome Bioinformatics
Lab Primig
Street address Klingelbergstr 50-70
City Basel
State/province Basel Stadt
ZIP/Postal code 4056
Country Switzerland
 
Platform ID GPL90
Series (1)
GSE1693 A novel response to microtubule perturbation in meiosis

Data table header descriptions
ID_REF
VALUE Normalized by variance stabilization (vsn), summarized using median polish, only perfect match. Exact procedure, see: http://www.bioz.unibas.ch/primig/benomyl/

Data table
ID_REF VALUE
10000_at 4.5871
10001_at 9.6658
10002_i_at 9.8913
10003_f_at 10.2088
10004_at 4.057
10005_at 7.7378
10006_at 7.131
10007_at 10.2366
10008_at 6.0452
10009_at 6.6971
10010_at 10.4136
10011_at 5.3504
10012_at 8.5641
10013_at 5.9737
10014_at 10.8069
10015_at 9.3831
10016_at 7.5463
10017_at 5.8325
10018_at 10.6118
10019_at 12.4887

Total number of rows: 9335

Table truncated, full table size 141 Kbytes.




Supplementary file Size Download File type/resource
GSM29080.CEL.gz 1.9 Mb (ftp)(http) CEL
GSM29080.EXP.gz 458 b (ftp)(http) EXP
GSM29080.RPT.gz 867 b (ftp)(http) RPT

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