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Sample GSM29089 Query DataSets for GSM29089
Status Public on Oct 18, 2004
Title SB8 - Replicate 2
Sample type RNA
Source name Total RNA
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
Description Cells were grown to saturation in YPD (YEP + 2% glucose) for 24 hours, diluted into YPA (YEP + 2% potassium acetate) at OD600= 0.3 and grown over night at 30 degree C. Cells were washed with sterilized water the next day and re-suspended in SPO medium (0.3% potassium acetate, pH = 7.0) at OD600= 1.9 at room temperature to induce sporulation. Benomyl [Methyl 1-(butylcarbamoyl)-2-benzimidazolecarbamate, Sigma-Aldrich; 30 mg/ml stock in DMSO] was dissolved to a final concentration of 0.4% in near-boiling SPO medium to avoid precipitation. The medium was then allowed to slowly cool to room temperature. 4 h after induction of sporulation cells were filtered and immediately re-suspended in medium containing Benomyl. The sample was taken 4 h after treatment with Benomyl.
Keywords = benomyl sporulation saccharomyces microtubule spindle checkpoint
Submission date Aug 18, 2004
Last update date May 27, 2005
Contact name Michael Primig
Phone +41 61 267 2098
Fax +41 61 267 3398
Organization name Biozentrum and Swiss Institute of Bioinformatics
Department Genome Bioinformatics
Lab Primig
Street address Klingelbergstr 50-70
City Basel
State/province Basel Stadt
ZIP/Postal code 4056
Country Switzerland
Platform ID GPL90
Series (1)
GSE1693 A novel response to microtubule perturbation in meiosis

Data table header descriptions
VALUE Normalized by variance stabilization (vsn), summarized using median polish, only perfect match. Exact procedure, see:

Data table
10000_at 4.5545
10001_at 9.3259
10002_i_at 9.5748
10003_f_at 9.7704
10004_at 4.1002
10005_at 7.4005
10006_at 6.5844
10007_at 10.0592
10008_at 5.8833
10009_at 6.3419
10010_at 10.2653
10011_at 5.5605
10012_at 8.3311
10013_at 5.9001
10014_at 10.8018
10015_at 9.8311
10016_at 7.8973
10017_at 5.4683
10018_at 11.7426
10019_at 12.1894

Total number of rows: 9335

Table truncated, full table size 141 Kbytes.

Supplementary file Size Download File type/resource
GSM29089.CEL.gz 1.9 Mb (ftp)(http) CEL
GSM29089.EXP.gz 456 b (ftp)(http) EXP
GSM29089.RPT.gz 868 b (ftp)(http) RPT

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