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Sample GSM2909394 Query DataSets for GSM2909394
Status Public on Feb 12, 2024
Title Non-tumor adult A2B5+ WM, sample 1
Sample type RNA
Source name Dissociated cells from normal adult WM, sorted on A2B5
Organism Homo sapiens
Characteristics tumor type: non-tumor (normal)
tumor grade: non-tumor (normal)
cell sort marker: A2B5+
developmental stage: adult
tissue source: white matter
Treatment protocol Tissue samples were dissociated with papain, cultured in suspension plates in serum-free media, and sorted MACS and FACS on A2B5 (tumor, adult normal samples) or CD140a (fetal normal samples).
Growth protocol N/A
Extracted molecule total RNA
Extraction protocol miRNA was extracted and purified with the Qiagen miRNeasy kit, according to the manufacturer's instructions.
Label biotin
Label protocol Biotin labeling was performed according to the manufacturer's instructions.
Hybridization protocol Hybridization was performed according to the manufacturer's instructions.
Scan protocol GeneChip scanning and image processing were performed according to the manufacturer's instructions.
Description A2B5_ADULT_WM_NONTUMOR_1
Small RNA
miRNA expression from Dissociated cells from normal adult WM, sorted on A2B5.
Data processing Raw intensity values were normalized using Variance Stabilizing Normalization (VSN) with RMA-style probeset summarization, as implemented by the vsnrma function of the VSN package in Bioconductor, in the R statistical software environment. As the Affymetrix miRNA 3.0 array platform includes miRNA for multiple species, prior to differential expression analysis data was filtered to include probes corresponding to mature human miRNAs.
Submission date Jan 02, 2018
Last update date Feb 12, 2024
Contact name Steven Goldman
Organization name University of Rochester Medical Center
Department Center for Translational Neuromedicine
Lab Goldman Lab
Street address 601 Elmwood Ave
City Rochester
State/province NY
ZIP/Postal code 14642
Country USA
Platform ID GPL16384
Series (1)
GSE255589 Human Adult Glial Progenitors Express Distinct miRNA Profiles Compared to their Fetal Counterparts

Data table header descriptions
VALUE Log2 intensity, after normalization with VSN and RMA-style background correction

Data table
14q0_st 3.341280332
14qI-1_st 4.269569749
14qI-1_x_st 3.732051761
14qI-2_st 2.651190565
14qI-3_x_st 2.979084276
14qI-4_st 3.473605008
14qI-4_x_st 3.828242011
14qI-5_st 2.625904582
14qI-6_st 2.061933686
14qI-6_x_st 1.903328376
14qI-7_st 4.6139641
14qI-8_st 2.372882334
14qI-8_x_st 2.364156005
14qI-9_x_st 2.454595908
14qII-1_st 6.432657822
14qII-1_x_st 6.383732451
14qII-10_st 2.673896089
14qII-10_x_st 2.58256985
14qII-11_st 2.483575727
14qII-11_x_st 1.935691979

Total number of rows: 25119

Table truncated, full table size 710 Kbytes.

Supplementary file Size Download File type/resource
GSM2909394_A2B5_ADULT_WM_NONTUMOR_1.CEL.gz 758.1 Kb (ftp)(http) CEL
Processed data included within Sample table

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